中国人膀胱癌组织中人乳头瘤病毒的流行率和整合状态。

Human Papillomavirus Prevalence and Integration Status in Tissue Samples of Bladder Cancer in the Chinese Population.

机构信息

Department of Urology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Department of Pathology, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

出版信息

J Infect Dis. 2021 Jul 2;224(1):114-122. doi: 10.1093/infdis/jiaa710.

DOI:10.1093/infdis/jiaa710

PMID:33205207

Abstract

BACKGROUND

Human papillomavirus (HPV) infection is associated with multiple types of cancer, but the evidence has not yet been fully elucidated in bladder cancer.

METHODS

Frozen tissue samples collected from 146 patients aged 32 to 89 years with bladder cancer pathological diagnosis between 2015 and 2019 were analyzed. HPV genotyping and integration status determination were performed by capture-based next generation sequencing. Statistical analysis of HPV type distributions was performed according to stage, grade, sex, and age group of patients.

RESULTS

Mean (SD) age of the 146 patients was 66.64 ± 10.06 years and 83.56% were men. Overall HPV infection rate was 28.77% (37.50% in women and 27.05% in men), with 11.90% HPV integration events. Among them, 17.12% single and 11.65% coinfections were observed. HPV18 (24.66%) was the most prevalent genotype, followed by HPV33, 16, and 39. All HPV were European lineage (A). HPV16 was more prevalent in women (P = .04).

CONCLUSIONS

HPV infection may contribute to the etiology both in men and women with bladder cancer. HPV18, followed by HPV33, 16, and 39 genotypes, potentially represent the predominant oncogenic risk types for bladder carcinogenesis.

摘要

背景

人乳头瘤病毒（HPV）感染与多种癌症有关，但在膀胱癌中的证据尚未完全阐明。

方法

分析了 2015 年至 2019 年间病理诊断为膀胱癌的 146 例年龄在 32 岁至 89 岁的患者的冷冻组织样本。采用基于捕获的下一代测序法进行 HPV 基因分型和整合状态检测。根据患者的分期、分级、性别和年龄组，对 HPV 型别分布进行统计学分析。

结果

146 例患者的平均（SD）年龄为 66.64±10.06 岁，83.56%为男性。总 HPV 感染率为 28.77%（女性为 37.50%，男性为 27.05%），HPV 整合事件发生率为 11.90%。其中，单感染占 17.12%，混合感染占 11.65%。HPV18（24.66%）是最常见的基因型，其次是 HPV33、16 和 39。所有 HPV 均为欧洲谱系（A）。HPV16 在女性中更为常见（P=0.04）。