Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Exp Parasitol. 2021 Jan;220:108042. doi: 10.1016/j.exppara.2020.108042. Epub 2020 Nov 15.
The aim of the current investigation was to assess the impacts of methanolic extract of Allium sativum (MEAS) on IL-4 (a cytokine derived from Th2 cells) and IFN-ɣ (a cytokine derived from Th1 cells) levels in mice infected with Echinococcus granulosus. Sixty healthy BALB/c female mice were used in this study. Each animal was intraperitoneally injected with 1500 protoscoleces. The infected animals were randomly divided into six groups: albendazole (100 mg/kg), MEAS 10 (10 mg/kg), MEAS 20 (20 mg/kg), MEAS 40 (40 mg/kg), MEAS 80 (80 mg/kg) and control group with no treatment. The studied animals received albendazole and/or MEAS through drinking water for 30 days. Serum IFN-γ concentration significantly increased in the MEAS 20 and 80 groups in comparison to the control, albendazole and MEAS 10 groups (P < 0.05). The serum IL-4 level showed no significant difference between the trial groups. The findings of this study showed that MEAS at 20 and 80 mg/kg concentrations enhanced Th1 cell response in mice with cystic echinococcosis.
本研究旨在评估大蒜甲醇提取物（MEAS）对感染细粒棘球蚴的小鼠白细胞介素 4（源自 Th2 细胞的细胞因子）和干扰素-γ（源自 Th1 细胞的细胞因子）水平的影响。本研究使用了 60 只健康的 BALB/c 雌性小鼠。每只动物均经腹腔注射 1500 个原头蚴。将感染的动物随机分为六组：阿苯达唑（100mg/kg）、MEAS10（10mg/kg）、MEAS20（20mg/kg）、MEAS40（40mg/kg）、MEAS80（80mg/kg）和无治疗的对照组。研究动物通过饮用水接受阿苯达唑和/或 MEAS 治疗 30 天。与对照组、阿苯达唑组和 MEAS10 组相比，MEAS20 和 80 组的血清 IFN-γ 浓度显著升高（P<0.05）。试验组之间的血清 IL-4 水平无显著差异。本研究结果表明，MEAS 在 20 和 80mg/kg 浓度下增强了囊型包虫病小鼠的 Th1 细胞反应。
