Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, GSP-1, Moscow 119991, Russia.
Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, GSP-1, Moscow 119991, Russia.
J Chromatogr A. 2020 Dec 20;1634:461648. doi: 10.1016/j.chroma.2020.461648. Epub 2020 Oct 29.
Novel anion exchangers with mono- and dialkanolamines in the external part of covalently-bonded hyperbranched functional layer were synthesized. Comparison of the chromatographic properties of the prepared stationary phases in suppressed ion chromatography (IC) mode using hydroxide eluent allowed us to evaluate the effect of the number of substituents at nitrogen atom in alkanolamine on selectivity of anion exchangers toward organic acids. Obtained anion exchangers were also examined together with previously described hyperbranched stationary phases with different mono- and diamines in the external part of the layer for evaluating the influence of various parameters on their selectivity. Effects of hydrophilicity, functionality of amine, and the number of substituents at nitrogen atom of amine used in the last modification cycle were established independently from each other, which provided the possibility to tailor selectivity toward organic acids when preparing anion exchanger for solving particular analytical task. Predominance of hydrophilicity as a key factor affecting the separation of weakly retained organic acids over other studied parameters was demonstrated.
合成了具有单烷醇胺和二烷醇胺的新型阴离子交换剂，它们位于共价键合的超支化功能层的外部。使用氢氧化物洗脱液在抑制型离子色谱（IC）模式下比较制备的固定相的色谱性能，使我们能够评估氮原子上取代烷醇胺的数量对阴离子交换剂对有机酸的选择性的影响。还研究了与先前在层的外部具有不同单胺和二胺的超支化固定相一起获得的阴离子交换剂，以评估各种参数对其选择性的影响。在最后一个修饰循环中使用的亲水性、胺的官能度和氮原子上取代基的数量的影响是相互独立的，这为在制备用于解决特定分析任务的阴离子交换剂时针对有机酸的选择性进行定制提供了可能性。证明了亲水性是影响弱保留有机酸分离的关键因素，超过了其他研究的参数。
