Jing He, Jin Mu, Li Yue, Sun Yuwei, Lu Jiakai, Cheng Weiping
Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, China.
Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
J Thorac Dis. 2020 Oct;12(10):5475-5484. doi: 10.21037/jtd-20-1966.
This is the first study to evaluate changes in postpartum pulmonary circulation in a novel pregnant rat model of monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH).
Female rats were randomly divided into two groups: the MCT-treated pregnant group, in which rats were injected with MCT (40 mg/kg) at the age of 7 weeks, and the pregnant group, in which rats were injected with the same volume of 0.9% saline at the same age. Rats from both groups were mated at the age of 9 weeks. General condition information, hemodynamic data and pulmonary tissues were collected from pregnant rats from the two groups on the 18th day after successful mating (T1) and the 1st (T2), 3rd (T3), and 7th days after delivery (T4).
The MCT-treated pregnant group exhibited a greater mean pulmonary artery pressure (mPAP) (P<0.01) and Fulton's Index (P<0.01) than the pregnant group at each time point. Lung tissues from the MCT-treated pregnant group showed pulmonary vascular hyperplasia and occlusive changes. The mPAP and the occluded pulmonary artery density in the MCT-treated pregnant group increased after delivery (P<0.01) and significantly increased at T3 compared with T2 (P<0.05) but was not further increased at T4 (P>0.05).
Pregnant rats with PAH exhibited increased mPAP after delivery accompanied by a significant increase in the occluded pulmonary artery density, which may have contributed to the increased mortality rate of pregnant rats with PAH after delivery.
这是第一项在新型的由野百合碱（MCT）诱导的肺动脉高压（PAH）妊娠大鼠模型中评估产后肺循环变化的研究。
将雌性大鼠随机分为两组：MCT处理的妊娠组，在7周龄时给大鼠注射MCT（40mg/kg）；妊娠组，在相同年龄给大鼠注射相同体积的0.9%生理盐水。两组大鼠均在9周龄时交配。在成功交配后第18天（T1）以及分娩后第1天（T2）、第3天（T3）和第7天（T4），收集两组妊娠大鼠的一般状况信息、血流动力学数据和肺组织。
在每个时间点，MCT处理的妊娠组的平均肺动脉压（mPAP）（P<0.01）和富尔顿指数（P<0.01）均高于妊娠组。MCT处理的妊娠组的肺组织显示肺血管增生和闭塞性改变。MCT处理的妊娠组的mPAP和闭塞肺动脉密度在分娩后增加（P<0.01），与T2相比，在T3时显著增加（P<0.05），但在T4时未进一步增加（P>0.05）。
患有PAH的妊娠大鼠在分娩后mPAP升高，同时闭塞肺动脉密度显著增加，这可能是导致患有PAH的妊娠大鼠分娩后死亡率增加的原因。
