[Over-expression of Hsa-miR-23b-3p suppresses proliferation, migration, invasion and epithelial-mesenchymal transition of human cervical cancer CasKi cells].

Objective To investigate the effects of miR-23b-3p on proliferation, migration and invasion of human cervical carcinoma CasKi cells. Methods Human cervical carcinoma CasKi cells and normal epithelial HaCaT cells were cultured in vitro. Real-time quantitative RT-PCR was conducted to detect the expression of miR-23b-3p in CasKi and HaCaT cells. Synthetic miR-23b-3p mimic and its negative control were transfected into CasKi cells by liposome method. The effects of miR-23b-3p over-expression on cell proliferation were detected by CCK-8 assay. Wound scratch healing assay and Transwell assay were used to observe the migration and invasion abilities of CasKi cells, respectively. Western blot analysis was used to detect the protein expression of N-cadherin, vimentin, E-cadherin, Snail, PCNA and cyclin D1. Results The expression of miR-23b-3p in CasKi cells was lower than that of HaCaT cells. Compared with the negative control group, the expression of miR-23b-3p were significantly up-regulated in CasKi cells after transfected with miR-23b-3p mimic. CCK-8 and Western blot assays showed that the proliferation was inhibited and the expression of PCNA and cyclin D1 were down-regulated after the cells were treated with miR-23b-3p mimic. At the same time, after over-expression of miR-23b-3p, the migration and invasion abilities of the CasKi cells were significantly inhibited. In addition, the expression of E-cadherin was up-regulated, while vimentin, Snail and N-cadherin expression levels were significantly down-regulated. Conclusion Over-expression of miR-23b-3p may suppress the proliferation, migration, invasion and epithelial-mesenchymal transition process of human cervical cancer CasKi cells.