Laboratory of Experimental Physiology, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.
Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.
Can J Physiol Pharmacol. 2021 Jul;99(7):720-728. doi: 10.1139/cjpp-2020-0339. Epub 2020 Nov 19.
The aim of the present study was to analyze the effect of neuromuscular electrical stimulation (NMES) and photobiomodulation (PBMT) on the cardiovascular parameters, hemodynamic function, arterial baroreflex sensitivity (BRS), and autonomic balance (ANS) of rats with heart failure (HF). Male Wistar rats (220-290 g) were organized into five groups: Sham ( = 6), Control-HF ( = 5), NMES-HF ( = 6), PBMT-HF ( = 6), and NMES + PBMT-HF ( = 6). Myocardial infarction (MI) was induced by left coronary artery ligation. Animals were subjected to an eight-week NMES and PBMT protocol. Statistical analysis included the General Linear Model (GLM) followed by a Bonferroni post-hoc test. Rats of the NMES-HF group showed a higher MI area than the Control-HF ( = 0.003), PBMT-HF ( = 0.002), and NMES + PBMT-HF ( = 0.012) groups. NMES-HF and NMES + PBMT-HF showed higher pulmonary congestion ( = 0.004 and = 0.02) and lower systolic pressure ( = 0.019 and = 0.002) than the Sham group. NMES + PBMT-HF showed lower mean arterial pressure ( = 0.02) than the Sham group. Control-HF showed a higher heart rate than the NMES-HF and NMES + PBMT-HF ( = 0.017 and = 0.013) groups. There was no difference in the BRS and ANS variables between groups. In conclusion, eight-week NMES isolated or associated with PBMT protocol reduced basal heart rate, systolic and mean arterial pressure, without influence on baroreflex sensibility and autonomic control, and no effect of PBMT was seen in rats with HF.
本研究旨在分析神经肌肉电刺激（NMES）和光生物调节（PBMT）对心力衰竭（HF）大鼠心血管参数、血液动力学功能、动脉压力反射敏感性（BRS）和自主平衡（ANS）的影响。雄性 Wistar 大鼠（220-290 克）分为五组：假手术组（ = 6）、对照组心衰组（ = 5）、NMES 心衰组（ = 6）、PBMT 心衰组（ = 6）和 NMES + PBMT 心衰组（ = 6）。通过左冠状动脉结扎诱导心肌梗死（MI）。动物接受了为期八周的 NMES 和 PBMT 方案。统计分析包括广义线性模型（GLM），随后进行 Bonferroni 事后检验。NMES 心衰组大鼠的 MI 面积高于对照组心衰组（ = 0.003）、PBMT 心衰组（ = 0.002）和 NMES + PBMT 心衰组（ = 0.012）。NMES 心衰组和 NMES + PBMT 心衰组的肺充血程度高于 Sham 组（ = 0.004 和 = 0.02），收缩压低于 Sham 组（ = 0.019 和 = 0.002）。NMES + PBMT 心衰组的平均动脉压低于 Sham 组（ = 0.02）。对照组心衰组的心率高于 NMES 心衰组和 NMES + PBMT 心衰组（ = 0.017 和 = 0.013）。各组间 BRS 和 ANS 变量无差异。总之，八周 NMES 单独或联合 PBMT 方案可降低基础心率、收缩压和平均动脉压，对压力反射敏感性和自主神经控制无影响，HF 大鼠中 PBMT 无作用。
