丛生福禄考愈伤组织悬浮细胞的生长和齐墩果酸积累。

Growth and Oleanolic Acid Accumulation of Polyscias fruticosa Cell Suspension Cultures.

机构信息

Department of Biotechnology, University of Sciences, Hue University, Hue, Vietnam.

出版信息

Curr Pharm Biotechnol. 2021;22(10):1266-1272. doi: 10.2174/1389201021666201119123503.

DOI:10.2174/1389201021666201119123503

PMID:33213337

Abstract

BACKGROUND

Oleanolic acid is an oleanane triterpene found in many plant species all over the world. This compound is also a major saponin in leaves of Polyscias fruticosa and possesses several promising pharmacological activities, such as hepatoprotective effects, and antiinflammatory, antioxidant, or anticancer activities.

OBJECTIVE

The objective of the present work is to establish cell suspension culture of P. fruticosa, investigate the influence of several factors such as plant growth regulators and carbon source on cell growth, and determine their oleanolic acid content.

METHODS

Cell culture was established by using 2 g fresh weight of 30 day old friable callus derived from in vitro stem segment in 50 mL of liquid medium with a shaking speed of 220 rpm. The culture was then incubated at 25±2ºC with a shaking speed of 120 rpm in the period of 12 h daylight at a light intensity of about 6.75 μmol/m2/s. Cell growth was measured by fresh and dry biomass at 16 h day. Oleanolic acid content was determined using HPLC analysis.

RESULTS & DISCUSSION: The study results showed that MS medium containing 2% sucrose as a carbon source, supplemented with 1 mg/L 6-benzylaminopurine and 0.5 mg/L 2,4-dichlorophenoxyacetic acid was the most appropriate growth medium. Cell biomass and oleanolic acid content reached the highest values of 0.43 g dry weight/flask and 25.4 mg/g dry weight, respectively.

CONCLUSION

These results indicated the potential production of oleanolic acid, a compound with high pharmacological value, from P. fruticosa cell culture.

摘要

背景

齐墩果酸是一种在世界各地许多植物物种中都存在的五环三萜酸。这种化合物也是圆柏枝叶中的一种主要皂角苷，具有多种有前途的药理活性，如护肝作用、抗炎、抗氧化或抗癌作用。

目的

本工作的目的是建立圆柏细胞悬浮培养体系，研究几种因素（如植物生长调节剂和碳源）对细胞生长的影响，并测定其齐墩果酸含量。

方法

采用 30 日龄易碎愈伤组织的 2 g 鲜重，在 50 mL 液体培养基中，以 220 rpm 的摇床速度进行细胞培养。在 25±2°C、120 rpm 摇床转速、12 h 光照/天、光照强度约为 6.75 μmol/m2/s 的条件下培养。在 16 h 天测量细胞生长情况，用鲜重和干重表示。采用高效液相色谱法（HPLC）分析齐墩果酸含量。