一种使用 Baudruche 膜（金箔）对成年雌性鸡皮刺螨（家禽红螨）进行体外饲养的改良方法。

An improved method for in vitro feeding of adult female Dermanyssus gallinae (poultry red mite) using Baudruche membrane (goldbeater's skin).

机构信息

Moredun Research Institute, Edinburgh, UK.

Scottish Rural Agricultural College, Edinburgh, UK.

出版信息

Parasit Vectors. 2020 Nov 19;13(1):585. doi: 10.1186/s13071-020-04471-x.

DOI:10.1186/s13071-020-04471-x

PMID:33213508

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7678122/

Abstract

BACKGROUND

Dermanyssus gallinae, or poultry red mite (PRM), is an important ectoparasite in laying hen, having a significant effect on animal welfare and potentially causing economic loss. Testing novel control compounds typically involves in vitro methodologies before in vivo assessments. Historically, in vitro methods have involved PRM feeding on hen blood through a membrane. The use of hen blood requires multiple procedures (bleeds) to provide sufficient material, and the use of a larger species (e.g. goose) could serve as a refinement in the use of animals in research.

METHODS

The in vitro feeding device used was that which currently employs a Parafilm™ M membrane (Bartley et al.: Int J Parasitol. 45:819-830, 2015). Adult female PMR were used to investigate any differences in mite feeding, egg laying and mortality when fed goose or hen blood. Effects on these parameters when PRM were fed through either the Parafilm™ M membrane or the Baudruche membrane alone or through a combination of the membrane with an overlaid polyester mesh were tested using goose blood.

RESULTS

Poultry red mites fed equally well on goose or hen blood through the Parafilm™ M membrane, and there were no significant differences in mortality of PRM fed with either blood type. A significant increase (t test: t = 3.467, df = 4, P = 0.03) in the number of eggs laid per fed mite was observed when goose blood was used. A 70% increase in PRM feeding was observed when the mites were fed on goose blood through a Baudruche membrane compared to when they were fed goose blood through the Parafilm™ M membrane. The addition of an overlaid polyester mesh did not improve feeding rates. A significant increase (analysis of variance: F = 3.193, P = 0.04) in PRM egg laying was observed in mites fed on goose blood through the Baudruche membrane compared to those fed goose blood through the Parafilm™ M membrane. A mean of 1.22 (standard error of the mean ± 0.04) eggs per fed mite was obtained using the Baudruche feeding device compared to only 0.87 (SEM ± 0.3) eggs per fed mite using the Parafilm™ M device when neither was combined with a polyester mesh overlay.

CONCLUSION

The in vitro feeding of adult female PRM can be readily facilitated through the use of goose blood in feeding devices with the Baudruche membrane.

摘要

背景

鸡皮刺螨（PRM）是一种重要的产蛋鸡外寄生虫，对动物福利有重大影响，并可能造成经济损失。测试新型控制化合物通常需要在体内评估之前进行体外方法。历史上，体外方法涉及 PRM 通过膜从母鸡血液中进食。使用母鸡血液需要多次采血程序以提供足够的材料，而使用较大的物种（例如鹅）可以作为动物在研究中使用的改进。

方法

使用的体外饲养装置是目前使用的 Parafilm™ M 膜（Bartley 等人：Int J Parasitol. 45:819-830, 2015）。成年雌性 PMR 被用于研究当喂食鹅或母鸡血液时，螨虫进食、产卵和死亡率的差异。使用鹅血测试了当 PRM 通过 Parafilm™ M 膜或 Baudruche 膜单独或通过膜与覆盖的聚酯网组合喂食时，这些参数的影响。

结果

PRM 通过 Parafilm™ M 膜同样可以从鹅或母鸡血液中进食良好，并且通过两种血液类型喂食的 PRM 死亡率没有显着差异。当使用鹅血时，每只喂食螨虫的产卵数显著增加（t 检验：t=3.467，df=4，P=0.03）。与通过 Parafilm™ M 膜喂食鹅血相比，当通过 Baudruche 膜喂食鹅血时，PRM 的进食量增加了 70%。在添加覆盖的聚酯网后，进食率没有提高。与通过 Parafilm™ M 膜喂食鹅血相比，当通过 Baudruche 膜喂食鹅血时，PRM 产卵的显著增加（方差分析：F=3.193，P=0.04）。与使用 Parafilm™ M 设备相比，使用 Baudruche 喂养装置，每只喂食螨虫的平均产卵数为 1.22（均值的标准误差±0.04），而使用 Parafilm™ M 设备时，每只喂食螨虫的平均产卵数仅为 0.87（SEM±0.3），并且都没有与聚酯网覆盖物结合使用。