Lalor P A, Mapp P I, Hall P A, Revell P A
Department of Morbid Anatomy, London Hospital Medical College, UK.
Rheumatol Int. 1987;7(5):183-6. doi: 10.1007/BF00541375.
The presence of proliferating cells has been sought in the synovium of rheumatoid arthritic (RA) and osteoarthritic (OA) joints using the monoclonal antibody Ki67, which marks a nuclear antigen present in all stages of the cell cycle apart from Go. Synovia were studied from 21 RA and 14 OA cases using the indirect immunoperoxidase technique. Double-staining was performed on 18 RA and 17 OA synovia with the simultaneous labelling of lysozyme (muramidase) by the immuno-alkaline phosphatase method and with Ki67 by the indirect immunoperoxidase method. Most of the RA and OA synovia showed an absence of Ki67-positive cells in the intimal cell layer. Three RA and four OA synovia showed no more than ten proliferating cells in the whole of the intimal layer examined. Similar results were obtained when double-labelling was performed. Eight RA and six OA synovia showed the presence of occasional Ki67-positive cells in the intimal layer. The total number of intimal cells was measured for each histological section, and the proliferation index calculated as the percentage of total cells in the intimal layer showing Ki67-positive staining. This varied between 0.03% and 0.0033% (between 1:2800 and 1:30,000 cells). In contrast, there were plentiful Ki67-positive cells present in the lymphocytic infiltrate and around blood vessels in the RA synovia and in the synovial infiltrate, where present, in the OA cases.(ABSTRACT TRUNCATED AT 250 WORDS)
利用单克隆抗体Ki67在类风湿性关节炎（RA）和骨关节炎（OA）关节滑膜中寻找增殖细胞，该抗体标记除G0期外细胞周期所有阶段均存在的一种核抗原。采用间接免疫过氧化物酶技术对21例RA和14例OA病例的滑膜进行研究。对18例RA和17例OA滑膜进行双重染色，采用免疫碱性磷酸酶法同时标记溶菌酶（胞壁质酶），采用间接免疫过氧化物酶法标记Ki67。大多数RA和OA滑膜在内膜细胞层未见Ki67阳性细胞。3例RA和4例OA滑膜在整个检查的内膜层中增殖细胞不超过10个。双重染色时也得到类似结果。8例RA和6例OA滑膜在内膜层偶见Ki67阳性细胞。对每个组织学切片的内膜细胞总数进行测量，并计算增殖指数，即内膜层显示Ki67阳性染色的细胞总数的百分比。其范围在0.03%至0.0033%之间（1:2800至1:30000个细胞之间）。相比之下，RA滑膜的淋巴细胞浸润区和血管周围以及OA病例滑膜浸润区（若存在）有大量Ki67阳性细胞。（摘要截短于250字）
