在卵巢刺激过程中，表达调控卵泡发育和成熟的基因在卵巢反应不良者中。

Expression of genes that regulate follicle development and maturation during ovarian stimulation in poor responders.

机构信息

Unit for Human Reproduction, Laboratory of Genetics, 1st Department of Obstetrics and Gynaecology, School of Medicine, Aristotle University of Thessaloniki, Papageorgiou General Hospital, Ring Road, Nea Efkarpia Thessaloniki 56403, Greece.

出版信息

Reprod Biomed Online. 2021 Jan;42(1):248-259. doi: 10.1016/j.rbmo.2020.05.012. Epub 2020 May 25.

DOI:10.1016/j.rbmo.2020.05.012

PMID:33214084

Abstract

RESEARCH QUESTION

Sex hormone-binding globulin (SHBG), androgen receptor (AR), LH beta polypeptide (LHB), progesterone receptor membrane component 1 (PGRMC1) and progesterone receptor membrane component 2 (PGRMC2) regulate follicle development and maturation. Their mRNA expression was assessed in peripheral blood mononuclear cells (PBMC) of normal and poor responders, during ovarian stimulation.

DESIGN

Fifty-two normal responders and 15 poor responders according to the Bologna criteria were enrolled for IVF and intracytoplasmic sperm injection and stimulated with 200 IU of follitrophin alpha and gonadotrophin-releasing hormone antagonist. HCG was administered for final oocyte maturation. On days 1, 6 and 10 of stimulation, blood samples were obtained, serum hormone levels were measured, RNA was extracted from PBMC and real-time polymerase chain reaction was carried out to identify the mRNA levels. Relative mRNA expression of each gene was calculated by the comparative 2 method.

RESULTS

Differences between mRNA levels of each gene on the same time point between the two groups were not significant. PGRMC1 and PGRMC2 mRNA levels were downregulated, adjusted for ovarian response and age. Positive correlations between PGRMC1 and AR (standardized beta = 0.890, P < 0.001) from day 1 to 6 and PGRMC1 and LHB (standardized beta = 0.806, P < 0.001) from day 1 to 10 were found in poor responders. PGRMC1 and PGRMC2 were positively correlated on days 6 and 10 in normal responders.

CONCLUSIONS

PGRMC1 and PGRMC2 mRNA are significantly decreased during ovarian stimulation, with some potential differences between normal and poor responders.

摘要

研究问题

性激素结合球蛋白（SHBG）、雄激素受体（AR）、黄体生成素β多肽（LHB）、孕激素受体膜成分 1（PGRMC1）和孕激素受体膜成分 2（PGRMC2）调节卵泡发育和成熟。在卵巢刺激期间，评估了正常和低反应者外周血单核细胞（PBMC）中的这些基因的 mRNA 表达。

设计

根据博洛尼亚标准，招募了 52 名正常反应者和 15 名低反应者进行 IVF 和胞浆内精子注射，并接受 200IU 的卵泡刺激素α和促性腺激素释放激素拮抗剂刺激。HCG 用于最终卵母细胞成熟。在刺激的第 1、6 和 10 天，采集血样，测量血清激素水平，从 PBMC 中提取 RNA，并进行实时聚合酶链反应以鉴定 mRNA 水平。通过比较 2 法计算每个基因的相对 mRNA 表达。

结果

两组在同一时间点的每个基因的 mRNA 水平之间没有差异。PGRMC1 和 PGRMC2 的 mRNA 水平下调，与卵巢反应和年龄调整。在低反应者中，PGRMC1 和 AR（标准化β=0.890，P<0.001）从第 1 天到第 6 天和 PGRMC1 和 LHB（标准化β=0.806，P<0.001）从第 1 天到第 10 天之间存在正相关。在正常反应者中，PGRMC1 和 PGRMC2 在第 6 天和第 10 天之间存在正相关。