Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
J Physiol Pharmacol. 2020 Aug;71(4). doi: 10.26402/jpp.2020.4.08. Epub 2020 Nov 15.
Transcellular calcium transport is an essential activity in mineralized tissue formation, including that in nervous systems. Dysregulation of Ca homeostasis can induce excitotoxicity and neurodegeneration in the central nervous system. Nckx3, a potassium-dependent Na/Ca exchanger, is most abundant in the brain and has a critical role in the transport of intracellular calcium across the cell membrane. However, the roles of Nckx3 in neuron development and function remain unreported. Herein, we examined the behaviors of Nckx3-knock-out mice at the age of six weeks. Detailed behavioral analyses showed Nckx3 mice exhibited an increase in moving distances in the open field test. Additionally, the rotarod test revealed motor learning defects in Nckx3 mice. Both Nckx3 and Nckx mice also exhibited deficits in sociability and social novelty preference. Furthermore, Nckx mice displayed increased depression-related behavior. However, there was no significant change in cognition function detected in Nckx mice. This study demonstrates that NCKX3 is involved in behavior and neuronal function.
细胞间钙转运是矿化组织形成的一种基本活动，包括神经系统中的矿化组织形成。钙稳态失调可导致中枢神经系统的兴奋性毒性和神经退行性变。Nckx3 是一种钾依赖的 Na/Ca 交换器，在大脑中含量最丰富，在跨细胞膜的细胞内钙转运中起关键作用。然而，Nckx3 在神经元发育和功能中的作用尚未报道。在此，我们研究了六周大的 Nckx3 敲除小鼠的行为。详细的行为分析表明，Nckx3 小鼠在旷场试验中移动距离增加。此外，转棒试验显示 Nckx3 小鼠存在运动学习缺陷。Nckx3 和 Nckx 小鼠在社交性和社交新颖性偏好方面也存在缺陷。此外，Nckx 小鼠表现出抑郁相关行为增加。然而，在 Nckx 小鼠中未检测到认知功能的显著变化。这项研究表明 NCKX3 参与了行为和神经元功能。
