Valdivia L A, Monden M, Gotoh M, Hasuike Y, Kubota N, Ichikawa T, Okamura J, Mori T
Second Department of Surgery, Osaka University Medical School, Japan.
Transplantation. 1987 Dec;44(6):759-63. doi: 10.1097/00007890-198712000-00007.
The immunosuppressive effect of splenectomy, alone or in combination with cyclosporine (CsA), was examined in hamster-to-rat orthotopic liver xenografts. The mean survival time was 7.3 +/- 0.5 days in untreated controls, 7.6 +/- 0.8 days with 40 mg/kg/day CsA, 7.2 +/- 0.4 days with splenectomy alone, and 17.6 +/- 5.6 days with splenectomy combined with 30 mg/kg/day CsA (P less than 0.01). The longest survival time was 27 days in this group. Marked enlargement of the spleen and high lymphocytotoxic antibody titer were characteristic of the unmodified recipients and those treated with CsA alone. Splenectomy by itself decreased the antibody formation without improvement of graft survival. In animals treated with the combined regimen, the lymphocytotoxic antibody titer was significantly suppressed, and the PMN and round cell infiltration were greatly reduced. Therefore, a synergistic effect was postulated between cyclosporine and splenectomy in this liver xenograft system.
在仓鼠到大鼠的原位肝异种移植中，研究了单独脾切除术或脾切除术联合环孢素（CsA）的免疫抑制作用。未治疗的对照组平均存活时间为7.3±0.5天，使用40mg/kg/天CsA的组为7.6±0.8天，单独脾切除术组为7.2±0.4天，脾切除术联合30mg/kg/天CsA组为17.6±5.6天（P<0.01）。该组最长存活时间为27天。未处理的受体及单独使用CsA处理的受体的特征是脾脏明显肿大和淋巴细胞毒性抗体滴度高。脾切除术本身可减少抗体形成，但不能改善移植物存活。在联合治疗的动物中，淋巴细胞毒性抗体滴度被显著抑制，中性粒细胞和圆形细胞浸润大大减少。因此，在这个肝异种移植系统中，推测环孢素和脾切除术之间存在协同作用。
