Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
J Sep Sci. 2021 Jan;44(2):636-644. doi: 10.1002/jssc.202000821. Epub 2020 Dec 29.
Polyethyleneimine-modified magnetic multi-walled carbon nanotubes were developed to extract 10 mycotoxins. Simple modification of polyethyleneimine was achieved on the magnetic substrate by using an epoxy-containing silane agent as a linker. The resultant magnetic adsorbent was integrated with reverse phase and anion exchange interaction sites. Under optimal extraction conditions, only 20.0 mg adsorbent was used to extract the mycotoxins from 50.0 mL loading solution. The maximum adsorption capacities of the adsorbent toward the mycotoxins ranged from 4.9 to 10.2 mg/g. Adsorption and desorption were completed within 3.0 and 2.0 min, respectively. The adsorbent could be used for six repeated runs without evident change in extraction performance. The adsorbent combined with liquid chromatography-tandem mass spectrometry was applied further to analyze the mycotoxins in milk. The absolute recoveries of the 10 mycotoxins ranged from 88.3 to 103.5% with relative standard deviations that ranged from 2.4 to 6.5%, and their limits of detection were 0.003 to 0.442 μg/kg. The proposed adsorbent has great potential in the routine analysis of mycotoxins in ordinary analytical chemistry laboratory.
聚乙二胺修饰的磁性多壁碳纳米管被开发用于提取 10 种真菌毒素。通过使用含环氧的硅烷试剂作为连接剂，在磁性基质上简单地修饰了聚乙二胺。所得的磁性吸附剂具有反相和阴离子交换相互作用位点。在最佳提取条件下，仅使用 20.0 mg 的吸附剂即可从 50.0 mL 的加载溶液中提取真菌毒素。该吸附剂对真菌毒素的最大吸附容量范围为 4.9 至 10.2 mg/g。吸附和解吸分别在 3.0 和 2.0 分钟内完成。该吸附剂可用于六次重复运行，而提取性能无明显变化。将吸附剂与液相色谱-串联质谱法进一步结合，用于分析牛奶中的真菌毒素。10 种真菌毒素的绝对回收率范围为 88.3%至 103.5%，相对标准偏差范围为 2.4%至 6.5%，检测限为 0.003 至 0.442 μg/kg。该提议的吸附剂在普通分析化学实验室中常规分析真菌毒素方面具有巨大的潜力。
