Laboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai An, China.
Plant Genome. 2020 Nov;13(3):e20052. doi: 10.1002/tpg2.20052. Epub 2020 Aug 19.
The TCP4 transcription factor plays an important role in plant growth and development, especially in flower development. PmTCP4 is involved in the process of pistil abortion in Japanese apricot, but its molecular mechanism, particularly the DNA binding sites and co-regulatory genes, are quite unknown. Therefore, to identify the genome-wide binding sites of PmTCP4 transcription factors and their co-regulatory genes, chromatin immunoprecipitation sequencing (ChIP-Seq) was carried out. ChIP-Seq data produced the maximum enriched peaks in two Japanese apricot cultivars 'Daqiandi' (DQD) and 'Longyan' (LY), which showed that the majority of DNA-protein interactions are relevant and have a significant function in binding sites. Moreover, 720 and 251 peak-associated genes regulated by PmTCP4 were identified in DQD and LY, respectively, and most of them were involved in the flower and pistil development process. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that photosynthesis and oxidative phosphorylation were the most enriched pathways in both cultivars and all identified genes related to these pathways were down-regulated. This study will provide a reference for a better understanding of the PmTCP4 regulatory mechanism during pistil abortion in Japanese apricot.
TCP4 转录因子在植物生长发育中发挥着重要作用，尤其是在花发育过程中。PmTCP4 参与了日本甜樱桃雌蕊败育的过程，但它的分子机制，特别是 DNA 结合位点和共调控基因，还知之甚少。因此，为了鉴定 PmTCP4 转录因子及其共调控基因的全基因组结合位点，进行了染色质免疫沉淀测序（ChIP-Seq）。ChIP-Seq 数据在两个日本甜樱桃品种‘大萁地’（DQD）和‘龙延’（LY）中产生了最大富集峰，这表明大多数 DNA-蛋白质相互作用是相关的，并且在结合位点具有重要功能。此外，在 DQD 和 LY 中分别鉴定到 720 个和 251 个受 PmTCP4 调控的峰相关基因，它们大多数参与花和雌蕊发育过程。此外，京都基因与基因组百科全书（KEGG）分析表明，光合作用和氧化磷酸化在两个品种中都是最丰富的途径，所有与这些途径相关的鉴定基因都下调。本研究将为更好地理解 PmTCP4 在日本甜樱桃雌蕊败育过程中的调控机制提供参考。
