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慢性多微生物性牙髓感染中的变形虫与毒力增加的微生物群落改变有关。

Amoebae in Chronic, Polymicrobial Endodontic Infections Are Associated with Altered Microbial Communities of Increased Virulence.

作者信息

Koller Garrit, Foschi Federico, Mitchell Philip, Witherden Elizabeth, Bruce Kenneth, Mannocci Francesco

机构信息

Department of Endodontics, Faculty of Dentistry, Oral & Craniofacial Sciences, Floor 22 Tower Wing, Guy's Dental Hospital, London SE1 9RT, UK.

Centre for Host Microbiome Interactions, King's College London Dental Institute at Guy's Hospital, King's Health Partners, London SE1 9RT, UK.

出版信息

J Clin Med. 2020 Nov 18;9(11):3700. doi: 10.3390/jcm9113700.

DOI:10.3390/jcm9113700
PMID:33218015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7698792/
Abstract

BACKGROUND

Infections of the root canal space involve polymicrobial biofilms and lead to chronic, low grade inflammatory responses arising from the seeding of microbes and by-products. Acute exacerbation and/or disseminating infections occur when established microbial communities undergo sudden changes in phenotypic behaviour.

METHODS

Within clinical endodontic infections, we assessedcategorical determinants comprising, and changing microbial composition of, chronic polymicrobial infections and their association with amoebae. After standardised assessment, primary or secondary infections underwent sampling and DNA processing, targeting bacteria, fungi and amoebae, including 16S high-throughput sequencing. After taxonomic assignment, community composition was correlated with clinical signs and symptoms. Diversity and abundance analyses were carried out in relation to the presence of non-bacterial amplicons.

RESULTS

Clinical specimens revealed two distinct community clusters, where specific changes correlated with clinical signs. An association between the compositions of microbiomes was found between these groups and the presence of in 44% of cases. When amoebae were present in endodontic infections, we demonstrate changes in microbial community structure that mirror those observed in treatment-resistant or recurrent infections.

CONCLUSIONS

Amoeba are present in endodontic infections at a high prevalence, and may promote increased virulence by enrichment for phagocytosis-resistant bacteria.

摘要

背景

根管系统感染涉及多种微生物形成的生物膜，并因微生物及其产物的植入引发慢性低度炎症反应。当已建立的微生物群落表型行为突然改变时，会发生急性加重和/或播散性感染。

方法

在临床牙髓感染中，我们评估了构成慢性多微生物感染并使其微生物组成发生变化的分类决定因素，以及它们与变形虫的关联。经过标准化评估后，对原发性或继发性感染进行采样和DNA处理，针对细菌、真菌和变形虫，包括16S高通量测序。在进行分类归属后，将群落组成与临床体征和症状进行关联分析。针对非细菌扩增子的存在进行多样性和丰度分析。

结果

临床标本显示出两个不同的群落簇，其中特定变化与临床体征相关。在这些组之间发现微生物群落组成存在关联，在44%的病例中与[此处原文缺失相关内容]的存在有关。当牙髓感染中存在变形虫时，我们证明微生物群落结构发生了变化，这与在治疗抵抗性或复发性感染中观察到的变化相似。

结论

变形虫在牙髓感染中普遍存在，并且可能通过富集抗吞噬细菌来促进毒力增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/81ee81a4eba7/jcm-09-03700-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/0842fab24e58/jcm-09-03700-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/380625472fbc/jcm-09-03700-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/d4fbf0123703/jcm-09-03700-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/b684b0d4ab2b/jcm-09-03700-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/b67d0e9795c0/jcm-09-03700-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/81ee81a4eba7/jcm-09-03700-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/0842fab24e58/jcm-09-03700-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/380625472fbc/jcm-09-03700-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/d4fbf0123703/jcm-09-03700-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/b684b0d4ab2b/jcm-09-03700-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/b67d0e9795c0/jcm-09-03700-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ab/7698792/81ee81a4eba7/jcm-09-03700-g006a.jpg

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本文引用的文献

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2
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Front Cell Infect Microbiol. 2018 Oct 29;8:379. doi: 10.3389/fcimb.2018.00379. eCollection 2018.
3
Pathogens, microbiome and the host: emergence of the ecological Koch's postulates.病原体、微生物组和宿主：生态科赫假设的出现。
FEMS Microbiol Rev. 2018 May 1;42(3):273-292. doi: 10.1093/femsre/fuy003.
4
MicrobiomeAnalyst: a web-based tool for comprehensive statistical, visual and meta-analysis of microbiome data.微生物组分析工具：一个基于网络的工具，用于对微生物组数据进行全面的统计、可视化和荟萃分析。
Nucleic Acids Res. 2017 Jul 3;45(W1):W180-W188. doi: 10.1093/nar/gkx295.
5
FISHing for gutta-percha-adhered biofilms in purulent post-treatment apical periodontitis.
Mol Oral Microbiol. 2017 Jun;32(3):226-235. doi: 10.1111/omi.12166. Epub 2016 Jul 29.
6
Amoeba-resisting bacteria found in multilamellar bodies secreted by Dictyostelium discoideum: social amoebae can also package bacteria.在盘基网柄菌分泌的多层小体中发现的抗变形虫细菌：社会性变形虫也能包裹细菌。
FEMS Microbiol Ecol. 2016 Mar;92(3). doi: 10.1093/femsec/fiw025. Epub 2016 Feb 8.
7
Macrophage cell activation with acute apical abscess contents determined by interleukin-1 Beta and tumor necrosis factor alpha production.通过白细胞介素-1β和肿瘤坏死因子α的产生来确定急性根尖脓肿内容物对巨噬细胞的激活作用。
J Endod. 2014 Nov;40(11):1752-7. doi: 10.1016/j.joen.2014.06.019. Epub 2014 Sep 6.
8
Detection of the amoeba Entamoeba gingivalis in periodontal pockets.牙周袋内牙龈内阿米巴的检测。
Parasite. 2014;21:30. doi: 10.1051/parasite/2014029. Epub 2014 Jul 2.
9
Trogocytosis by Entamoeba histolytica contributes to cell killing and tissue invasion.溶组织内阿米巴的细胞胞饮作用有助于细胞杀伤和组织侵袭。
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10
Comparison of the bacterial composition and structure in symptomatic and asymptomatic endodontic infections associated with root-filled teeth using pyrosequencing.运用焦磷酸测序技术比较根管充填后牙齿有症状和无症状牙髓感染中的细菌组成及结构。
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