Howell L P, Zipfel S, Steplewski Z, Koprowska I
Department of Pathology, Temple University School of Medicine, Philadelphia, PA 19140.
Acta Cytol. 1987 Nov-Dec;31(6):802-6.
A new monoclonal antibody (MAb), 29-10, produced by immunization of mice with cells from the SW 1116 colorectal carcinoma cell line, detected an antigen present in cytologic touch imprints of surgically resected normal and neoplastic gastrointestinal (GI) tissue, including specimens from the stomach and the colon. These imprints were fixed in 95% ethanol and stained with the avidin-biotin immunoperoxidase technique. In tested cases, 22 (100%) of 22 imprints from GI adenocarcinomas and from normal GI tissue, as well as 13 (56.6%) of 23 imprints from colonic polyps, stained positively while no staining was demonstrable in imprints from other tissues. In histologic sections, only 4 (23%) of 17 colonic adenocarcinomas and 3 (11.5%) of 26 polyps stained positively. The staining ability of MAb 29-10 was compared to that of MAb 19-9, another colorectal antibody, and was found to be markedly superior for binding of the antigen in cytologic preparations. This tissue-specific antibody may be useful in identifying malignant cells of metastatic carcinoma as to their GI tract origin.
用SW 1116结肠癌细胞系的细胞免疫小鼠产生的一种新的单克隆抗体（MAb）29-10，能检测出手术切除的正常和肿瘤性胃肠道（GI）组织的细胞学压印片中存在的一种抗原，包括来自胃和结肠的标本。这些压印片用95%乙醇固定，并用抗生物素蛋白-生物素免疫过氧化物酶技术染色。在测试病例中，来自胃肠道腺癌和正常胃肠道组织的22张压印片中的22张（100%），以及来自结肠息肉的23张压印片中的13张（56.6%）呈阳性染色，而其他组织的压印片中未显示染色。在组织学切片中，17例结肠腺癌中只有4例（23%）和26例息肉中只有3例（11.5%）呈阳性染色。将单克隆抗体29-10的染色能力与另一种结肠直肠抗体单克隆抗体19-9的染色能力进行比较，发现其在细胞学制剂中结合抗原的能力明显更强。这种组织特异性抗体可能有助于鉴定转移性癌的恶性细胞的胃肠道起源。
