Guo Yanmei, Wang Huiqi, Zhang Feifei, Xia Jianfei, Wang Zonghua
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, PR China.
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, PR China.
Anal Chim Acta. 2020 Dec 15;1140:10-17. doi: 10.1016/j.aca.2020.10.010. Epub 2020 Oct 7.
Enzyme cascade reactions can greatly improve catalytic efficiency and achieve selective and sensitive detection of targets. This paper presents a novel strategy for colorimetric sensing of targets by loading natural enzymes into a hemin-doped G-quadruplex (G4-hemin) biohydrogel to form a flexible enzyme cascade sensing (FECS) platform. The biohydrogel has advantages of biocompatibility, printability and flexibility. The biohydrogel not only participates in the cascade reaction as the mimic enzyme but also provides a mild microenvironment for the natural enzyme. The FECS platform has a linear range from 0.4 μM to 120 μM and a detection limit of 3.6 × 10 M for hydrogen peroxide detection. Additionally, the FECS platform has a low detection limit and wide linear range for glucose and xanthine by loading xanthine oxidase (XOD) and glucose oxidase (GOD) into the biohydrogel, respectively. These results indicate that the novel FECS platform is an effective detection platform that can detect multiple targets and is expected to be widely used in flexible sensing.
酶级联反应能够极大地提高催化效率，并实现对目标物的选择性和灵敏检测。本文提出了一种通过将天然酶负载到血红素掺杂的G-四链体（G4-血红素）生物水凝胶中以形成柔性酶级联传感（FECS）平台来比色传感目标物的新策略。该生物水凝胶具有生物相容性、可打印性和柔韧性等优点。该生物水凝胶不仅作为模拟酶参与级联反应，还为天然酶提供温和的微环境。FECS平台在检测过氧化氢时线性范围为0.4 μM至120 μM，检测限为3.6×10⁻⁷ M。此外，通过分别将黄嘌呤氧化酶（XOD）和葡萄糖氧化酶（GOD）负载到生物水凝胶中，FECS平台对葡萄糖和黄嘌呤也具有低检测限和宽线性范围。这些结果表明，新型FECS平台是一种能够检测多种目标物的有效检测平台，有望在柔性传感中得到广泛应用。
