Lloyd R V
Department of Pathology, University of Michigan Medical Center, Ann Arbor.
Am J Pathol. 1987 Dec;129(3):441-7.
The reverse hemolytic plaque assay (RHPA) was used to detect hormone secretion from normal pituitary cells and from the transplantable MtT/F4 pituitary tumor cells. Aliquots of the same cell suspensions were analyzed by immunocytochemistry (ICC). Normal pituitaries had more growth hormone (GH)-producing cells than tumors when analyzed by both the RHPA and ICC. However, the MtT/F4 tumor had significantly more prolactin (PRL)-secreting cells. Mammosomatotropic (MS) cells, which produced both PRL and GH, were identified in both normal and tumorous pituitaries with the RHPA and ICC. A combined procedure of RHPA followed by ICC staining on the same slide also revealed MS cells in both normal and tumorous pituitary cells, although the percentage of MS with this technique was less than with the other two methods. These results show that MS cells from a significant population of cells in the MtT/F4 tumor and that the RHPA and ICC can be used to study the regulation of this cell type.
反向溶血空斑试验（RHPA）用于检测正常垂体细胞和可移植的MtT/F4垂体肿瘤细胞的激素分泌。通过免疫细胞化学（ICC）分析相同细胞悬液的等分试样。当通过RHPA和ICC分析时，正常垂体产生生长激素（GH）的细胞比肿瘤更多。然而，MtT/F4肿瘤分泌催乳素（PRL）的细胞明显更多。通过RHPA和ICC在正常和肿瘤性垂体中均鉴定出产生PRL和GH的促乳腺生长素（MS）细胞。在同一张载玻片上先进行RHPA然后进行ICC染色的联合程序也在正常和肿瘤性垂体细胞中发现了MS细胞，尽管用这种技术检测到的MS细胞百分比低于其他两种方法。这些结果表明，MS细胞存在于MtT/F4肿瘤的大量细胞中，并且RHPA和ICC可用于研究这种细胞类型的调节。
