Fyfe L, Coleman G, Munro A L
Department of Biochemistry, Nottingham University Medical School, UK.
Ann Inst Pasteur Microbiol (1985). 1986 Mar-Apr;137A(2):117-23. doi: 10.1016/s0769-2609(86)80016-3.
Aeromonas salmonicida was grown aerobically and anaerobically in supplemented 3% (w/v) tryptone soya broth medium for 24 h at 25 degrees C. Although the bacterial density achieved was 4.9 times higher in the aerobic culture, the exoprotein produced per unit of bacterial dry weight was only 1.9 times higher than in the anaerobic culture. However, the protease activity of the exoprotein showed a marked reduction anaerobically, being only one-tenth of that of the exoprotein produced aerobically. This finding was consistent with the differing SDS-PAGE patterns of the extracellular proteins from the two cultures, which also showed marked loss and reinforcement of other, as yet unidentified extracellular products.
嗜水气单胞菌在添加了3%（w/v）胰蛋白胨大豆肉汤的培养基中，于25℃下分别进行有氧培养和厌氧培养24小时。尽管有氧培养获得的细菌密度比厌氧培养高4.9倍，但每单位细菌干重产生的胞外蛋白仅比厌氧培养高1.9倍。然而，胞外蛋白的蛋白酶活性在厌氧条件下显著降低，仅为有氧培养产生的胞外蛋白的十分之一。这一发现与两种培养物胞外蛋白不同的SDS-PAGE图谱一致，该图谱也显示出其他尚未鉴定的胞外产物有明显的减少和增强。
