Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha 410208, Hunan, China.
Aging (Albany NY). 2020 Nov 18;12(23):23945-23959. doi: 10.18632/aging.104068.
Alzheimer's disease (AD) is an age-related neurodegenerative disease with a high incidence worldwide, and with no medications currently able to prevent the progression of AD. Danggui-Shaoyao-San (DSS) is widely used in traditional Chinese medicine (TCM) and has been proven to be effective for memory and cognitive dysfunction, yet its precise mechanism remains to be delineated. The present study was designed to investigate the genome-wide expression profile of long non-coding RNAs (LncRNAs) and messenger RNAs (mRNAs) in the hippocampus of APP/PS1 mice after DSS treatment by RNA sequencing. A total of 285 differentially expressed LncRNAs and 137 differentially expressed mRNAs were identified (fold-change ≥2.0 and < 0.05). Partial differentially expressed LncRNAs and mRNAs were selected to verify the RNA sequencing results by quantitative polymerase chain reaction (qPCR). A co-expression network was established to analyze co-expressed LncRNAs and genes. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to evaluate the biological functions related to the differentially co-expressed LncRNAs, and the results showed that the co-expressed LncRNAs were mainly involved in AD development from distinct origins, such as APP processing, neuron migration, and synaptic transmission. Our research describes the lncRNA and mRNA expression profiles and functional networks involved in the therapeutic effect of DSS in APP/PS1 mice model. The results suggest that the therapeutic effect of DSS on AD involves the expression of LncRNAs. Our findings provide a new perspective for research on the treatment of complex diseases using traditional Chinese medicine prescriptions.
阿尔茨海默病（AD）是一种与年龄相关的神经退行性疾病，在全球范围内发病率较高，目前尚无药物能够阻止 AD 的进展。当归芍药散（DSS）在中药中被广泛应用，已被证明对记忆和认知功能障碍有效，但确切的机制仍需阐明。本研究旨在通过 RNA 测序研究 DSS 处理 APP/PS1 小鼠海马组织中长链非编码 RNA（LncRNA）和信使 RNA（mRNA）的全基因组表达谱。共鉴定出 285 个差异表达的 LncRNA 和 137 个差异表达的 mRNA（fold-change≥2.0且<0.05）。选择部分差异表达的 LncRNA 和 mRNA 通过定量聚合酶链反应（qPCR）验证 RNA 测序结果。建立共表达网络分析共表达的 LncRNA 和基因。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析评估与差异共表达 LncRNA 相关的生物学功能，结果表明，共表达的 LncRNA 主要参与 AD 从不同起源的发展，如 APP 加工、神经元迁移和突触传递。本研究描述了 DSS 在 APP/PS1 小鼠模型中治疗作用涉及的 LncRNA 和 mRNA 表达谱和功能网络。结果表明，DSS 对 AD 的治疗作用涉及 LncRNA 的表达。我们的发现为使用中药方剂治疗复杂疾病的研究提供了新的视角。
