免疫调节细胞因子表达与马疱疹病毒 9 脑炎仓鼠模型的组织病理学变化和病毒抗原的相关性。
Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis.
机构信息
Department of Pathology, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt; Neuroscience Laboratory, CHU de Québec Research Centre, Department of Molecular Medicine, Faculty of Medicine, Laval University, Canada.
出版信息
J Comp Pathol. 2020 Oct;180:46-54. doi: 10.1016/j.jcpa.2020.07.007. Epub 2020 Sep 15.
A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correlated with the severity of sequential histopathological changes and intensity of immunohistochemical labelling of virus antigen in brain. Early and progressive upregulation of all the proinflammatory and anti-inflammatory cytokines investigated (P < 0.05) was correlated with increasing severity of encephalitis and viral antigen expression from 2 days post infection (dpi) with a peak at 4-5 dpi (P <0.05).
一组仓鼠（n=25）经鼻腔感染马疱疹病毒 9 型（EHV-9），使用 RT-qPCR 检测脑组织中几种促炎（IFN-γ、TNF-α 和 IL-6）和抗炎（IL-4、IL-10 和 TGF-β）细胞因子的 mRNA 转录水平。这些水平与脑组织中病毒抗原的组织病理学变化严重程度和免疫组化标记强度相关。所有研究的促炎和抗炎细胞因子均呈早期和进行性上调（P<0.05），与从感染后 2 天（dpi）开始的脑炎严重程度和病毒抗原表达逐渐增加相关，在 4-5dpi 时达到峰值（P<0.05）。
Zotero 插件