Gholami Ali, Bahrami Fahimeh, Faraji Mohammad
Department of Analytical Chemistry, Faculty of Science, University of Kashan, Kashan, Iran.
Department of Food Science and Technology, Faculty of Food Industry and Agriculture, Standard Research Institute (SRI), Karaj, Iran.
Iran J Pharm Res. 2020 Spring;19(2):274-282. doi: 10.22037/ijpr.2019.111659.13292.
A simple, rapid, and ultra sensitive dispersive solid phase extraction based on nano graphene oxide was developed for simultaneous measurement of trace amounts of metformin (MET) and linagliptin (LIN) in plasma samples by HPLC-UV-Vis. Affecting factors on the extraction of these drugs, including adsorbent weight, extraction time, organic solvent type, desorption situations, and composition of solvent were examined and optimized. In optimum conditions, the LOD (limit of detection) and LOQ (limit of quantification) of the suggested technique were 2.0 ngmL and 6.1 (ngmL) for LIN and 3.0 ngmL and 9.2 ngmL for MET, respectively. Suitable linear behavior in the considered ranges of concentration (10-2000 ngmL) and good correlation coefficient of 0.9901 and 0.9903 (r) for LIN and MET were obtained, respectively. The RSD (relative standard deviations) according to three replicate measurements at 2, 20, 200 ngmL levels of these drugs was less than 8.0%. In the last step, applicability of the suggested technique was examined by analyzing the drugs in plasma samples and reasonable results were achieved.
基于纳米氧化石墨烯开发了一种简单、快速且超灵敏的分散固相萃取方法，用于通过高效液相色谱-紫外-可见分光光度法同时测定血浆样品中的痕量二甲双胍（MET）和利那格列汀（LIN）。考察并优化了影响这些药物萃取的因素，包括吸附剂重量、萃取时间、有机溶剂类型、解吸情况和溶剂组成。在最佳条件下，该方法对LIN的检测限（LOD）和定量限（LOQ）分别为2.0 ng/mL和6.1 ng/mL，对MET分别为3.0 ng/mL和9.2 ng/mL。在10 - 2000 ng/mL的浓度范围内获得了合适的线性行为，LIN和MET的相关系数（r）分别为0.9901和0.9903。在2、20、200 ng/mL水平下对这些药物进行三次重复测量的相对标准偏差（RSD）小于8.0%。最后，通过分析血浆样品中的药物考察了该方法的适用性，并获得了合理的结果。
