Ortiz Isabel, Dorado Jesús, Morrell Jane M, Diaz-Jimenez Maria Angeles, Pereira Blasa, Consuegra César, Hidalgo Manuel
Veterinary Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, Spain.
Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Anim Reprod. 2019 Oct 23;16(2):282-289. doi: 10.21451/1984-3143-AR2018-0133.
The aim of this study was to compare the post-thaw distribution of motile sperm subpopulations, following simple or colloid centrifugation. A new analysis was used to evaluate the available number of sperm from each subpopulation after each centrifugation protocol. Frozen/thawed semen samples were divided into the following after-thawing treatments: uncentrifuged control (UDC), sperm washing (SW) and two colloid centrifugation procedures (Equipure, SLC-E, and Androcoll, SLC-A). Percentage of total and progressive motility (TM and PM), as well as sperm motility kinematics, distribution of motile sperm subpopulations, and recovery rates, were statistically compared among treatments. The SLC treatments showed higher (P < 0.001) TM and PM than UDC and SW. Following each SLC procedure, different percentages of the subpopulation with the most vigorous and progressive sperm (sP4) were obtained. SLC-A recovered a larger number of sperm belonging to sP4 than SLC-E, but not significantly higher than SW. From a practical point of view, sperm washing, the standard centrifugation procedure for equine semen processing, recovered the same amount of fast and progressive sperm as colloid centrifugation, apparently the best treatment according to traditional analysis. In conclusion, samples processed by SLC have higher motility percentages than SW and UDC but, after combining the available number of sperm, SLC and SW techniques are equally efficient in recovering sperm from the most vigorous, fast and progressive motile subpopulation (sP4).
本研究的目的是比较简单离心或胶体离心后活动精子亚群的解冻后分布情况。采用一种新的分析方法来评估每种离心方案后各亚群的可用精子数量。冷冻/解冻后的精液样本解冻后分为以下处理组：未离心对照组（UDC）、精子洗涤组（SW）和两种胶体离心程序（Equipure，SLC-E，以及Androcoll，SLC-A）。对各处理组的总活力和前向运动活力百分比（TM和PM）、精子运动学、活动精子亚群分布以及回收率进行了统计学比较。SLC处理组的TM和PM高于UDC和SW组（P < 0.001）。在每个SLC程序之后，获得了不同百分比的活力最强且前向运动的精子亚群（sP4）。SLC-A回收的属于sP4的精子数量比SLC-E多，但并不显著高于SW组。从实际角度来看，精子洗涤作为马精液处理的标准离心程序，回收的快速前向运动精子数量与胶体离心相同，显然根据传统分析这是最佳处理方法。总之，SLC处理的样本活力百分比高于SW组和UDC组，但综合可用精子数量来看，SLC和SW技术在从活力最强、快速前向运动的活动亚群（sP4）中回收精子方面同样有效。
