Deaciuc I V, Spitzer J A
Louisiana State University Medical Center Department of Physiology New Orleans 70112.
Cell Calcium. 1987 Oct;8(5):365-76. doi: 10.1016/0143-4160(87)90011-x.
The hypothesis was tested that several metabolic and functional alterations associated with endotoxicosis and sepsis could be due to a Ca overload of liver and heart in rats. Male rats were given intravenously E. coli endotoxin (ET) or rendered septic by cecal ligation and puncture. At various intervals after ET injection and 16-18 h after the onset of peritonitis the animals were sacrificed and the liver and heart sampled for assay of the total Ca content. Also, livers of saline- or ET-treated rats were perfused in vitro to study several aspects of Ca movements as affected by phenylephrine infusion. Livers, were also fractionated to study the subcellular distribution of Ca. ET treatment produced a slight, but significant increase in total hepatic Ca content (12.6% at 4 h and 7.7% at 24 h after ET injection). Sepsis did not affect this parameter in either liver or heart. ET also produced a decrease of Ca content in the microsomal fraction of liver, while sepsis was associated with an elevated Ca content of liver mitochondria. Perfused livers of saline-treated rats responded to phenylephrine by accumulating Ca, while the perfused organs of ET-treated rats did not display such a response to agonist infusion. We conclude that impairment of intracellular Ca homeostasis--under the conditions studied--consists of a slight Ca overload in endotoxicosis, associated with discrete alterations of Ca fluxes and compartmentalization within the cell both in endotoxicosis and sepsis.
与内毒素血症和脓毒症相关的几种代谢和功能改变可能是由于大鼠肝脏和心脏的钙超载所致。给雄性大鼠静脉注射大肠杆菌内毒素（ET）或通过盲肠结扎和穿刺使其发生脓毒症。在注射ET后的不同时间间隔以及腹膜炎发作后16 - 18小时，处死动物并采集肝脏和心脏样本以测定总钙含量。此外，对生理盐水或ET处理的大鼠肝脏进行体外灌注，以研究去氧肾上腺素输注对钙转运几个方面的影响。肝脏也进行了分级分离以研究钙的亚细胞分布。ET处理使肝脏总钙含量略有但显著增加（注射ET后4小时增加12.6%，24小时增加7.7%）。脓毒症对肝脏或心脏的这个参数没有影响。ET还使肝脏微粒体部分的钙含量降低，而脓毒症与肝脏线粒体钙含量升高有关。生理盐水处理的大鼠灌注肝脏对去氧肾上腺素的反应是积累钙，而ET处理的大鼠灌注器官对激动剂输注没有显示出这样的反应。我们得出结论，在所研究的条件下，细胞内钙稳态的损害在内毒素血症中表现为轻微的钙超载，与内毒素血症和脓毒症中细胞内钙通量和分隔的离散改变有关。
