Comprehensive Microbe Resources Lab B, College of Life Science, Shihezi University, Shihezi, 832000, Xinjiang, China.
Arch Microbiol. 2021 Apr;203(3):1175-1182. doi: 10.1007/s00203-020-02112-6. Epub 2020 Nov 23.
To understand the relationship between the community structure of bacteria and soil environment, the diversity and composition of soil bacterial communities were investigated, based on 16S rRNA gene clone library, in three different sampling sites (SP1, SP2 and SP3) in the Bortala and Jinghe River basins of Ebinur Lake Wetland. The results showed that the diversity of bacteria among plots was SP2 > SP3 > SP1, and the richness was SP3 > SP2 > SP1. Community composition analysis of bacteria showed that Proteobacteria and Bacteroides accounted for 49.7% and 53.7%, respectively, making them the most dominant phyla observed. In SP1, Proteobacteria was the most dominant phylum, followed by Bacteroides. In SP2 and SP3, Bacteroides was the most dominant phylum, followed by Proteobacteria. At subphyla level, Gammaproteobacteria, Alphaproteobacteria and Deltaproteobacteria accounted for 50%, 51.0%, and 42.2% of the Proteobacteria of SP1, SP2, and SP3, respectively. Betaproteobacteria and Epsilonproteobacteria were found only in SP3. RDA results showed that SOM, SM and EC were the main soil environmental factors affecting bacterial community structure.
为了了解细菌群落结构与土壤环境之间的关系,本研究基于 16S rRNA 基因克隆文库,对艾比湖湿地博尔塔拉河和精河干流流域的三个不同采样点(SP1、SP2 和 SP3)的土壤细菌群落的多样性和组成进行了调查。结果表明,各采样点间细菌多样性表现为 SP2>SP3>SP1,丰富度表现为 SP3>SP2>SP1。细菌群落组成分析表明,变形菌门和拟杆菌门分别占 49.7%和 53.7%,是最主要的两个门类。在 SP1 中,变形菌门是最主要的门类,其次是拟杆菌门。在 SP2 和 SP3 中,拟杆菌门是最主要的门类,其次是变形菌门。在亚门水平上,γ-变形菌门、α-变形菌门和δ-变形菌门分别占 SP1、SP2 和 SP3 变形菌门的 50%、51.0%和 42.2%。β-变形菌门和ε-变形菌门仅在 SP3 中被发现。RDA 结果表明,SOM、SM 和 EC 是影响细菌群落结构的主要土壤环境因素。