Rodriguez M, Alonso L, Alvarez E, Blázquez E
Departamento of Bioquímica, Facultad de Medicina, Salamanca, Spain.
Diabetologia. 1987 Oct;30(10):820-2. doi: 10.1007/BF00275750.
To determine the effect of insulin on its own receptor concentrations in circulating monocytes of normal human adults and full-term newborns, these cells were chronically exposed (2-18 h) to 10(-9) to 10(-7) mol/l insulin at 37 degrees C in vitro. The reduced 125I-insulin binding observed in monocytes from adult individuals was dependent on the concentration of unlabelled insulin and on the duration of exposure, while in the monocytes obtained from umbilical cord blood, insulin did not induce any reduction of insulin binding. The modifications observed in insulin binding were accounted for by changes in receptor concentrations rather than by any change in receptor affinity for the hormone. These findings show that insulin does not down-regulate its own receptors in monocytes of human newborns.
为了确定胰岛素对正常成年人及足月新生儿循环单核细胞中自身受体浓度的影响，将这些细胞在体外37℃下长期（2 - 18小时）暴露于10^(-9)至10^(-7)摩尔/升的胰岛素中。在成年个体的单核细胞中观察到的125I - 胰岛素结合减少取决于未标记胰岛素的浓度和暴露持续时间，而在从脐带血获得的单核细胞中，胰岛素并未诱导胰岛素结合的任何减少。胰岛素结合中观察到的变化是由受体浓度的变化引起的，而不是受体对激素的亲和力发生任何变化。这些发现表明，胰岛素不会下调人类新生儿单核细胞中的自身受体。
