新生儿中的胰岛素受体。受体亲和力和数量增加。

Insulin receptors in the newborn. Increase in receptor affinity and number.

作者信息

Thorsson A V, Hintz R L

出版信息

N Engl J Med. 1977 Oct 27;297(17):908-12. doi: 10.1056/NEJM197710272971704.

DOI:10.1056/NEJM197710272971704

Abstract

Insulin hs been implicated as a growth factor in fetal life. To clarify the role of insulin in the fetus, we studied insulin receptors on mononuclear leukocytes in placental-cord blood from 12 normal newborns. Eight healthy young adults served as controls. Specific binding (mean +/- S.E.M.) in the absence of unlabeled insulin per 10(7) monocytes was, respectively, 24.3+/-3.5 and 4.7+/-0.9 per cent in newborns and adults. This increase in binding is due to a rise in number of receptor sites per cell (newborns, 44,600, and adults, 7100) as well as an increase in receptor affinity (Ke for newborns = 5.9 X 10(8) M(-1), and that for adults = 2.9 X 10(8) M(-1). This finding of markedly high concentrations of high-affinity receptors for insulin on fetal cells supports the importance of insulin in intrauterine growth and development.

摘要

胰岛素已被认为是胎儿期的一种生长因子。为阐明胰岛素在胎儿体内的作用，我们研究了12名正常新生儿胎盘脐带血中单核白细胞上的胰岛素受体。8名健康青年成年人作为对照。在无未标记胰岛素的情况下，每10⁷个单核细胞的特异性结合（平均值±标准误）在新生儿和成年人中分别为24.3±3.5%和4.7±0.9%。结合的这种增加是由于每个细胞受体位点数量的增加（新生儿为44,600个，成年人是7100个）以及受体亲和力的增加（新生儿的Kd = 5.9×10⁻⁸ M⁻¹，成年人的为2.9×10⁻⁸ M⁻¹）。胎儿细胞上存在高浓度的高亲和力胰岛素受体这一发现支持了胰岛素在子宫内生长发育中的重要性。