Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Food Chem. 2021 May 15;344:128641. doi: 10.1016/j.foodchem.2020.128641. Epub 2020 Nov 16.
In this study, effects of alcalase on physical properties, pepsin digestibility, and antioxidative activity in the heat-induced surimi gel were investigated to develop a novel gel product for people with dysphagia. The heat-induced gelation profile of surimi showed that alcalase activity was maximized at approximately 37 °C. The surimi gel attained an appropriate texture for people with dysphagia, when a combination of 0.3-0.5% alcalase and two-step heating at 37 °C and 90 °C was used. Adding alcalase effectively promoted proteolysis, resulting in softening of the gel. Furthermore, the gel with 0.5% alcalase showed improved pepsin digestibility, when heated at 37 °C and 90 °C. Its antioxidative activity was enhanced by adding 0.5% alcalase. Therefore, a combination of 0.5% alcalase and the two-step heating at 37 °C and 90 °C was useful in improving the physical and functional properties of the surimi gel for people with dysphagia.
在这项研究中,研究了碱性蛋白酶对热诱导鱼糜凝胶物理性质、胃蛋白酶消化率和抗氧化活性的影响,旨在开发一种适合吞咽困难人群的新型凝胶产品。鱼糜的热诱导凝胶形成曲线表明,碱性蛋白酶的活性在大约 37°C 时达到最大值。当使用 0.3-0.5%的碱性蛋白酶和 37°C 和 90°C 的两步加热组合时,鱼糜凝胶获得了适合吞咽困难人群的适当质地。添加碱性蛋白酶可有效促进蛋白质水解,使凝胶变软。此外,当在 37°C 和 90°C 下加热时,添加 0.5%碱性蛋白酶可提高凝胶的胃蛋白酶消化率。添加 0.5%碱性蛋白酶可增强其抗氧化活性。因此,0.5%碱性蛋白酶和 37°C 和 90°C 的两步加热的组合可有效改善吞咽困难人群的鱼糜凝胶的物理和功能特性。