Bo Jian-Zhu, Xue Ling, Li Shuang, Yin Jing-Wen, Li Zheng-Yao, Wang Xi, Wang Jun-Feng, Zhang Yan-Shu
College of Public Health, North China University of Science and Technology, Tangshan, Hebei Province, China.
Laboratory Animal Center, North China University of Science and Technology, Tangshan, Hebei Province, China.
Neural Regen Res. 2021 May;16(5):836-841. doi: 10.4103/1673-5374.297086.
Although exogenous D-serine has been applied as a neural regulatory intervention in many studies, the role played by D-serine in hippocampal injuries caused by lead exposure remains poorly understood. Rat models of chronic lead exposure were established through the administration of 0.05% lead acetate for 8 weeks. Simultaneously, rats were administered 30 or 60 mg/kg D-serine, intraperitoneally, twice a day. Our results showed that D-serine treatment shortened the escape latency from the Morris water maze, increased the number of times that mice crossed the original platform location, and alleviated the pathological damage experienced by hippocampal neurons in response to lead exposure. Although D-serine administration did not increase the expression levels of the N-methyl-D-aspartate receptor subtype 2B (NR2B) in the hippocampi of lead-exposed rats, 60 mg/kg D-serine treatment restored the expression levels of NR2A, which are reduced by lead exposure. These findings suggested that D-serine can alleviate learning and memory impairments induced by lead exposure and that the underlying mechanism is associated with the increased expression of NR2A in the hippocampus. This study was approved by the Animal Ethics Committee of North China University of Science and Technology, China (approval No. LX2018155) on December 21, 2018.
尽管在许多研究中，外源性D-丝氨酸已被用作一种神经调节干预手段，但D-丝氨酸在铅暴露所致海马损伤中所起的作用仍知之甚少。通过给予0.05%醋酸铅8周建立慢性铅暴露大鼠模型。同时，大鼠腹腔注射30或60mg/kg D-丝氨酸，每日两次。我们的结果表明，D-丝氨酸治疗缩短了Morris水迷宫的逃避潜伏期，增加了小鼠穿过原平台位置的次数，并减轻了海马神经元因铅暴露而遭受的病理损伤。虽然给予D-丝氨酸并没有增加铅暴露大鼠海马中N-甲基-D-天冬氨酸受体2B亚型（NR2B）的表达水平，但60mg/kg D-丝氨酸治疗恢复了因铅暴露而降低的NR2A的表达水平。这些发现表明，D-丝氨酸可以减轻铅暴露诱导的学习和记忆障碍，其潜在机制与海马中NR2A表达增加有关。本研究于2018年12月21日获得中国华北理工大学动物伦理委员会批准（批准号：LX2018155）。
