Gisina A M, Kim Ya S, Gabashvili A N, Tsvetkova A V, Vakhrushev I V, Yarygin K N, Lupatov A Yu
V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
National University of Science and Technology MISIS, Moscow, Russia.
Bull Exp Biol Med. 2020 Nov;170(1):135-141. doi: 10.1007/s10517-020-05018-x. Epub 2020 Nov 24.
We studied the formation of spheroids by Caco-2, SW480, and HCT116 human colorectal adenocarcinoma cell lines under low-adhesion culturing conditions. Of these three cell lines, only HCT116 formed stable tumor spheroids. Flow cytometry analysis of 19 surface markers in monolayer HCT116 culture and spheroids formed by these cells revealed considerable similarity of the expression profiles in these two culturing modes. The only exception was EpCAM molecule: its expression in spheroids was 3-fold higher than in the monolayer culture. Scanning confocal laser microscopy showed equal EpCAM distribution in the inner mass of the spheroids.
我们研究了Caco-2、SW480和HCT116人结肠直肠腺癌细胞系在低粘附培养条件下球体的形成。在这三种细胞系中，只有HCT116形成了稳定的肿瘤球体。对单层HCT116培养物和由这些细胞形成的球体中的19种表面标志物进行流式细胞术分析，结果显示这两种培养模式下的表达谱具有相当的相似性。唯一的例外是EpCAM分子：其在球体中的表达比单层培养物中高3倍。扫描共聚焦激光显微镜显示EpCAM在球体内部的分布均匀。
