College of Forestry, Northwest A&F University, Yangling, Shaanxi, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources (South China Agricultural University), College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China.
Arch Insect Biochem Physiol. 2021 Feb;106(2):e21760. doi: 10.1002/arch.21760. Epub 2020 Nov 24.
The Chinese white pine beetle Dendroctonus armandi (Tsai and Li) is a significant pest of the Qinling and Bashan Mountains pine forests of China. The Chinese white pine beetle can overcome the defences of Chinese white pine Pinus armandi (Franch) through pheromone-assisted aggregation that results in a mass attack of host trees. We isolated five full-length complementary DNAs encoding mevalonate pathway-related enzyme genes from the Chinese white pine beetle (D. armandi), which are acetoacetyl-CoA thiolase (AACT), geranylgeranyl diphosphate synthase (GGPPS), mevalonate kinase (MK), mevalonate diphosphate decarboxylase (MPDC), and phosphomevalonate kinase (PMK). Bioinformatic analyses were performed on the full-length deduced amino acid sequences. Differential expression of these five genes was observed between sexes, and within these significant differences among topically applied juvenile hormone III (JH III), fed on phloem of P. armandi, tissue distribution, and development stage. Mevalonate pathway genes expression were induced by JH III and feeding.
马尾松毛虫(Dendroctonus armandi)是中国秦巴山区马尾松森林的重要害虫。马尾松毛虫通过信息素辅助聚集，可以克服马尾松的防御，导致大量攻击宿主树木。我们从中国白松毛虫(D. armandi)中分离出五个全长编码甲羟戊酸途径相关酶基因的 cDNA，分别是乙酰乙酰辅酶 A 硫解酶(AACT)、香叶基香叶基二磷酸合酶(GGPPS)、甲羟戊酸激酶(MK)、甲羟戊酸二磷酸脱羧酶(MPDC)和磷酸甲羟戊酸激酶(PMK)。对全长推导的氨基酸序列进行了生物信息学分析。这些五个基因在性别之间存在差异表达，并且在体内有明显的差异，包括局部应用的保幼激素 III (JH III)、取食马尾松韧皮部、组织分布和发育阶段。甲羟戊酸途径基因的表达受到 JH III 和取食的诱导。
