State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
College of Forestry, Northwest A&F University, No. 3 Taicheng Road, Yangling, Xianyang 712100, China.
Int J Mol Sci. 2022 Mar 2;23(5):2749. doi: 10.3390/ijms23052749.
Allatostatin C (PISCF/AST) is a neuropeptide gene that affects juvenile hormone (JH) synthesis in the corpora allata. Juvenile hormone acid O-methyltransferase () is a key gene in the JH biosynthetic pathway. In this study, two genes encoding and were cloned. Both and were expressed in the larvae, pupae and adults of Chinese white pine beetle (), and highly expressed in the head and the gut. The expression of the two genes was induced by JH analog (JHA) methoprene and the functions of the two genes were then investigated by RNAi. Considering the role of hormones in metamorphosis, JHA significantly induced and in the larval stage. knockdown in larvae, pupae and adults significantly increased the mRNA levels. Moreover, knockdown of instead of increased pupae mortality and the abnormal rate of emergence morphology and reduced emergence rates. However, knockdown of instead of significantly reduced frontalin biosynthesis in adult males. The results showed that acts as an allatostatin and inhibits JH biosynthesis, and that is a key regulatory enzyme for JH synthesis in the .
Allatostatin C (PISCF/AST) 是一种影响前胸腺中保幼激素 (JH) 合成的神经肽基因。保幼激素酸 O-甲基转移酶 () 是 JH 生物合成途径中的关键基因。在这项研究中，克隆了编码 和 的两个基因。 和 均在华山松大小蠹幼虫、蛹和成虫中表达，且在头部和肠道中高度表达。两种基因的表达均受到 JH 类似物 (JHA) 灭幼脲的诱导，随后通过 RNAi 研究了两种基因的功能。考虑到激素在变态中的作用，JHA 显著诱导了幼虫阶段的 和 。幼虫、蛹和成虫中 的敲低显著增加了 的 mRNA 水平。此外，敲低 而不是 会增加蛹的死亡率以及出孔形态异常率，并降低出孔率。然而，敲低 而不是 会显著减少成年雄性前胸腺中的法尼醇生物合成。结果表明， 作为一种脑肠肽抑制 JH 生物合成， 是 中 JH 合成的关键调节酶。
