Department of Chemistry, Texas A&M University, 580 Ross Street, College Station, Texas 77845, United States.
J Am Soc Mass Spectrom. 2021 Sep 1;32(9):2288-2295. doi: 10.1021/jasms.0c00356. Epub 2020 Nov 24.
In this study, we develop an alternating current (AC)-induced electro-epoxidation reaction and incorporate it into nanoelectrospray ionization for locating carbon-carbon double-bonds in positively and negatively charged forms of lipids simultaneously. An AC voltage plays multiple roles in this method, including initiation of the electro-epoxidation of carbon-carbon double-bonds in both charged states of lipids and protonation/deprotonation of lipids for detection in both ion modes. Moreover, the rapid switch between native lipids and their electro-epoxidation products can be achieved at different AC voltages. The efficacy of the present method was demonstrated in mixtures of lipid standards and in a biological polar lipid extract. The advantages of simultaneous detection of negatively and positively charged unsaturated lipids, the low sample consumption, and on-demand electro-epoxidation should allow its wide applications in lipid-related research.
在本研究中，我们开发了一种交流电（AC）诱导的电环氧化反应，并将其与纳升电喷雾电离结合，同时定位脂质的正电荷和负电荷形式中的碳-碳双键。在这种方法中，交流电电压发挥了多种作用，包括引发脂质的正电荷和负电荷状态下的碳-碳双键的电环氧化以及脂质的质子化/去质子化以在两种离子模式下进行检测。此外，可以在不同的交流电电压下实现天然脂质与其电环氧化产物之间的快速切换。本方法在脂质标准混合物和生物极性脂质提取物中的应用证明了其有效性。同时检测带负电荷和带正电荷的不饱和脂质的优势、低样品消耗以及按需电环氧化应该使其在脂质相关研究中得到广泛应用。
