Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Laboratório de Alimentos Funcionais, Rio de Janeiro 22290-240, Brazil.
Universidade Federal Fluminense (UFF), Faculdade de Veterinária, Niterói 24230-340, Brazil.
Food Res Int. 2020 Nov;137:109450. doi: 10.1016/j.foodres.2020.109450. Epub 2020 Jun 17.
The present study aimed to evaluate the antiproliferative and apoptotic effects of probiotic whey dairy beverages in human prostate cancer cell lines (PC-3 and DU-145). Five different whey beverages were manufactured: conventional whey beverage (without addition of probiotic strain, CTL); and whey beverages containing Lactobacillus acidophilus La-05, Lactobacillus acidophilus La-03, Lactobacillus casei-01, and Bifidobacterium animalis Bb-12. Cell viability was determined by MTT assay and cell cycle arrest, and apoptosis by flow cytometry. All the samples presented cytotoxic activities against both cell lines. A decrease in the percentage of PC-3 cells in G/G and S, followed by an increase in G/M phase were observed with L. casei-01, Bb-12 and La-05 beverages (50.0 and 100.0 µg/mL). The extracts of the whey beverages caused extensive apoptosis induction in both cells' lines, regardless of the probiotic strain. However, the whey beverage added with L. casei-01 might be a better candidate against prostate cancer cells.
本研究旨在评估益生菌乳清乳制品对人前列腺癌细胞系（PC-3 和 DU-145）的抗增殖和促凋亡作用。制备了五种不同的乳清饮料：常规乳清饮料（未添加益生菌菌株，CTL）；以及含有嗜酸乳杆菌 La-05、嗜酸乳杆菌 La-03、干酪乳杆菌-01 和双歧杆菌 Bb-12 的乳清饮料。通过 MTT 测定法和流式细胞术检测细胞活力、细胞周期阻滞和细胞凋亡。所有样品对两种细胞系均表现出细胞毒性作用。用 L. casei-01、Bb-12 和 La-05 饮料（50.0 和 100.0μg/mL）处理后，PC-3 细胞的 G/G 和 S 期百分比下降，G/M 期百分比增加。乳清饮料的提取物可诱导两种细胞系的广泛凋亡，而与益生菌菌株无关。然而，添加 L. casei-01 的乳清饮料可能是一种更好的前列腺癌细胞候选物。
