Pimentel-Moral S, Borrás-Linares I, Lozano-Sánchez J, Alañón M E, Arráez-Román D, Segura-Carretero A
Research and Development Functional Food Centre, Health Science Technological Park, Avda Conocimiento n° 37, C.P. 18016 Granada, Spain; Department of Analytical Chemistry. Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, C.P. 18071 Granada, Spain.
Research and Development Functional Food Centre, Health Science Technological Park, Avda Conocimiento n° 37, C.P. 18016 Granada, Spain.
Food Res Int. 2020 Nov;137:109466. doi: 10.1016/j.foodres.2020.109466. Epub 2020 Jun 22.
An environmental friendly extraction procedure has been tested to extract phenolic compounds from H. sabdariffa calyces using pressurized GRAS solvents. A central composite rotatable design (CCRD) was performed to evaluate the influence of the main operational conditions: temperature (40-200 °C) and solvent composition based on aqueous hidroalcoholic solutions (0-100% ethanol). Phenolic composition of experimental extracts analyzed by HPLC-ESI-QTOF-MS showed that higher temperatures and greater ethanol percentages drove to solvents with lower dielectric constants, which resulted in extracts with major quantities of phenolic compounds. An exception was the extraction of cyanidin-3-sambubioside that could only be quantified in extracts performed at the lowest temperature (40 °C) due to its thermal sensibility. In addition, a RSM was carried out with the aim to maximize the extraction of total phenolic content. To this end, the predicted optimal extraction conditions by RSM were 200 °C and 100% (v/v) of ethanol. Results showed that temperature and ethanol percentage had a significant influence on the extraction of total phenolic compounds (p value < 0.05). The mathematical model pointed out 200 °C of temperature and 100% of ethanol as the optimum conditions to perform the isolation of phenolic compounds by means of pressurized GRAS solvents.
已测试了一种环保提取方法，使用加压的公认安全（GRAS）溶剂从玫瑰茄花萼中提取酚类化合物。进行了中心复合旋转设计（CCRD）以评估主要操作条件的影响：温度（40 - 200°C）和基于水 - 乙醇溶液的溶剂组成（0 - 100%乙醇）。通过HPLC - ESI - QTOF - MS分析的实验提取物的酚类组成表明，较高的温度和较高的乙醇百分比会导致溶剂的介电常数较低，从而得到含有大量酚类化合物的提取物。一个例外是矢车菊素 - 3 - 接骨木二糖苷的提取，由于其热敏感性，只能在最低温度（40°C）下进行的提取物中进行定量。此外，进行了响应面法（RSM）以最大化总酚含量的提取。为此，RSM预测的最佳提取条件为200°C和100%（v/v）乙醇。结果表明，温度和乙醇百分比对总酚类化合物的提取有显著影响（p值<0.05）。数学模型指出，200°C的温度和100%的乙醇是使用加压GRAS溶剂分离酚类化合物的最佳条件。
