分散的大鼠垂体前叶细胞中促黄体生成素及其β亚基的分泌特征

The characteristics of luteinizing hormone and its beta-subunit secretion in dispersed rat anterior pituitary cells.

作者信息

Seki T, Makino T, Tanimoto S, Takahashi M, Iida T, Iizuka R

机构信息

Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo.

出版信息

Nihon Sanka Fujinka Gakkai Zasshi. 1987 Dec;39(12):2180-4.

PMID:3323364

Abstract

Secretion of luteinizing hormone (LH) and its beta-subunit (LH-beta) by luteinizing hormone-releasing hormone (LH-RH) were studied in perifused rat anterior pituitary cells. LH release was dose related between 10(-10) and 10(-6)M LH-RH. Every 5-minute pulse of 10(-8)M LH-RH induced almost similar response to the initial LH release; neither the self-priming effect nor desensitization were significant. Continuous administration of 10(-8)M LH-RH caused an initial LH release, followed by distinct desensitization, which could not be overcome by subsequent pulses of 10(-8)M LH-RH. The results were quite similar in LH-beta release. The perifusion study is shown to be useful in analyzing the characteristics of LH and LH-beta release, and the present results suggest that LH-RH receptor-mediated regulation may be also involved in LH-beta release.

摘要

在经灌流的大鼠垂体前叶细胞中，研究了促黄体生成激素释放激素（LH-RH）对促黄体生成激素（LH）及其β亚基（LH-β）分泌的影响。在10^(-10)至10^(-6)M的LH-RH浓度范围内，LH释放呈剂量依赖性。每5分钟给予一次10^(-8)M的LH-RH脉冲，诱导的反应与最初的LH释放几乎相似；既没有明显的自身启动效应，也没有明显的脱敏现象。持续给予10^(-8)M的LH-RH会引起最初的LH释放，随后出现明显的脱敏，随后再给予10^(-8)M的LH-RH脉冲也无法克服这种脱敏现象。LH-β释放的结果与之非常相似。灌流研究被证明有助于分析LH和LH-β释放的特性，目前的结果表明，LH-RH受体介导的调节可能也参与了LH-β的释放。