Infectious Disease Research Institute, 1616 Eastlake Ave E, Seattle, WA, USA.
Infectious Disease Research Institute, 1616 Eastlake Ave E, Seattle, WA, USA; Department of Global Health, University of Washington, Seattle, WA, USA.
J Chromatogr A. 2021 Jan 4;1635:461705. doi: 10.1016/j.chroma.2020.461705. Epub 2020 Nov 13.
QS-21 is a triterpene glycoside saponin found in the bark of the Chilean soap bark tree Quillaja saponaria. It is a highly potent vaccine adjuvant that is included in two approved vaccines and has shown promise in numerous other vaccine candidates in the research and clinical pipelines. One major hurdle to the widespread use of this adjuvant is the difficulty of obtaining it in high yield and purity. Previously reported purification approaches either showed suboptimal purity and/or yield, lacked efficiency, or had strict requirement on the composition of the starting material. Here, we report the development of a new two-step orthogonal chromatographic process, consisting of a polar reversed-phase (RP) chromatography step followed by a hydrophilic interaction chromatography (HILIC) step, for purifying QS-21 from a commercially available Quillaja saponaria bark extract with high yield and > 97% purity. This process makes available a simple and efficient method for obtaining highly pure QS-21 from saponin-enriched bark extract.
QS-21 是一种三萜糖苷皂苷，存在于智利皂树 Quillaja saponaria 的树皮中。它是一种高效的疫苗佐剂，包含在两种已批准的疫苗中，并在研究和临床管道中的许多其他疫苗候选物中显示出前景。该佐剂广泛应用的一个主要障碍是难以高产率和高纯度地获得它。以前报道的纯化方法要么显示出不理想的纯度和/或产率，要么缺乏效率，或者对起始材料的组成有严格的要求。在这里，我们报告了一种新的两步正交色谱过程的开发，该过程由极性反相（RP）色谱步骤和亲水相互作用色谱（HILIC）步骤组成，用于从市售的 Quillaja saponaria 树皮提取物中以高产率和＞97%的纯度纯化 QS-21。该过程提供了一种从富含皂苷的树皮提取物中获得高纯度 QS-21 的简单高效方法。
