Curative Inc., San Dimas, California, USA.
Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering, University of Southern California, Los Angeles, California, USA.
J Clin Microbiol. 2021 Jan 21;59(2). doi: 10.1128/JCM.02510-20.
Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We have evaluated an antibody detection approach using the OraSure Technologies oral antibody collection device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure test for total antibody detection in oral fluid had comparable sensitivity and specificity to commercially available serum-based ELISAs for SARS-CoV-2 antibody detection while allowing for a more accessible form of specimen collection with the potential for self-collection.
目前，由于需要由经过高度训练的采血员使用个人防护设备（PPE）采集全血，因此可靠地检测针对严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的抗体的商业上可获得的方法仍然很昂贵且难以获得。我们已经评估了一种使用 OraSure 技术口腔抗体采集装置（OACD）和其专有的 SARS-CoV-2 总抗体检测酶联免疫吸附测定（ELISA）的抗体检测方法。我们发现，OraSure 检测口腔液中的总抗体检测与商业上可用于 SARS-CoV-2 抗体检测的基于血清的 ELISA 具有相当的敏感性和特异性，同时允许更易于获得的标本采集方式，并且具有自我采集的潜力。
