From the Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine; and the Department of Plastic and Reconstructive Surgery, Vrije Universiteit Brussel, Universitair Ziekenhuis Brussel.
Plast Reconstr Surg. 2021 Feb 1;147(2):373-385. doi: 10.1097/PRS.0000000000007580.
Fat graft ischemia impedes us from having satisfying long-term results. The quality and quantity culture is a 1-week cell culture that increases the vasculogenic potential of peripheral blood mononuclear cells (PBMNC). This in vivo murine model investigates whether enrichment with quality and quantity-cultured human mononuclear cells (MNC-QQ) improves the vascularization in the human fat graft and whether this decreases the tissue loss.
Human adipose tissue, PBMNC, MNC-QQ, and stromal vascular fraction were prepared. First, PBMNC, MNC-QQ, and stromal vascular fraction were compared in vitro for vasculogenic potential by endothelial progenitor cell colony-forming and culture assays. Second, 0.25-g fat grafts were created with 1 × 106 PBMNC (n = 16), 1 × 106 MNC-QQ (n = 16), 1 × 106 stromal vascular fraction (n = 16), or phosphate-buffered saline as control (n = 16) before grafting in BALB/c nude mice. Grafts were analyzed for weight persistence, vessel formation by CD31 immunohistochemistry, and angiogenic markers by quantitative polymerase chain reaction.
MNC-QQ develop more definitive endothelial progenitor cell colonies and more functional endothelial progenitor cells compared to PBMNC and stromal vascular fraction. Weight persistence after 7 weeks was significantly higher in grafts with MNC-QQ (89.8 ± 3.5 percent) or stromal vascular fraction (90.1 ± 4.2 percent) compared with control (70.4 ± 6.3 percent; p < 0.05). MNC-QQ-enriched grafts had the highest vessel density (96.6 ± 6.5 vessels/mm2; control, 70.4 ± 5.6 vessels/mm2; p < 0.05). MNC-QQ exerted a direct vasculogenic effect through vascular integration and a potential paracrine vascular endothelial growth factor-mediated effect.
Quality and quantity-cultured human mononuclear cells containing endothelial progenitor cells stimulate fat graft vascularization and enhance graft survival in a rodent recipient.
脂肪移植缺血阻碍了我们获得满意的长期效果。质量和数量培养是一种为期一周的细胞培养，可增加外周血单核细胞（PBMNC）的血管生成潜能。本体内小鼠模型研究了富含质量和数量培养的人单核细胞（MNC-QQ）是否改善了人脂肪移植物的血管化，以及是否减少了组织损失。
准备人脂肪组织、PBMNC、MNC-QQ 和基质血管部分。首先，通过内皮祖细胞集落形成和培养测定比较 PBMNC、MNC-QQ 和基质血管部分的体外血管生成潜能。其次，在 BALB/c 裸鼠中创建 0.25 克脂肪移植物，每个移植物中包含 1×106 PBMNC（n=16）、1×106 MNC-QQ（n=16）、1×106 基质血管部分（n=16）或磷酸盐缓冲盐水作为对照（n=16）。分析移植物的重量持久性、CD31 免疫组织化学的血管形成和定量聚合酶链反应的血管生成标记物。
与 PBMNC 和基质血管部分相比，MNC-QQ 形成更明确的内皮祖细胞集落和更具功能的内皮祖细胞。7 周后，MNC-QQ（89.8±3.5%）或基质血管部分（90.1±4.2%）的移植物重量持久性明显高于对照组（70.4±6.3%；p<0.05）。MNC-QQ 富集的移植物血管密度最高（96.6±6.5 个血管/mm2；对照组为 70.4±5.6 个血管/mm2；p<0.05）。MNC-QQ 通过血管整合发挥直接血管生成作用，并通过潜在的旁分泌血管内皮生长因子介导作用。
富含内皮祖细胞的质量和数量培养的人单核细胞刺激脂肪移植物血管化，并增强啮齿动物受者中移植物的存活。
