Zhang Huishan, Wu Shuzhen, Ye Shaoxin, Ma Huiting, Liu Zhengping
Department of Fetal Medicine Research, Foshan Fetal Medicine Research Institute, Foshan Women and Children's Hospital Affiliated to Southern Medical University, Foshan, Guangdong 528000, P.R. China.
Department of Obstetrics, Foshan Women and Children's Hospital Affiliated to Southern Medical University, Foshan, Guangdong 528000, P.R. China.
Exp Ther Med. 2021 Jan;21(1):13. doi: 10.3892/etm.2020.9445. Epub 2020 Nov 4.
Long non-coding RNAs (lncRNAs) are reported to have important roles in placental development and function, but the role of lncRNAs in abnormally invasive placenta (AIP) remains elusive. In the present study, the differential expression profiles of lncRNAs were analyzed to identify novel targets for further study of AIP. A total of 10 lncRNAs were chosen for validation by reverse transcription-quantitative PCR. To further determine the functions of dysregulated lncRNAs and their corresponding mRNAs, functional enrichment analysis, coexpression analysis were performed. A total of 329 lncRNAs and 179 mRNAs were identified to be differently expressed between the invasive and control group. Gene ontology analysis revealed that the 10 most significantly enriched functions included upregulated mRNAs and the most significantly enriched term was related to the proteinaceous extracellular matrix (ECM). In the pathway analysis, the two most significantly enriched pathways were the TGF-β signaling pathway for upregulated mRNAs and the pentose phosphate pathway for downregulated mRNAs. Furthermore, for certain dysregulated lncRNAs, their associated mRNAs were also dysregulated. Of note, BMP and activin membrane-bound inhibitor and TGF-β-induced, as the target genes of the TGF-β pathway, were indicated to be closely related to the ECM and invasive placental cells. Their nearby lncRNAs G008916 and vault RNA2-1 were also significantly dysregulated. In conclusion, significant lncRNAs with the potential to serve as biomarkers for AIP were identified.
据报道，长链非编码RNA（lncRNAs）在胎盘发育和功能中发挥重要作用，但lncRNAs在异常侵袭性胎盘（AIP）中的作用仍不清楚。在本研究中，分析了lncRNAs的差异表达谱，以确定用于AIP进一步研究的新靶点。共选择了10个lncRNAs通过逆转录定量PCR进行验证。为了进一步确定失调的lncRNAs及其相应mRNA的功能，进行了功能富集分析和共表达分析。在侵袭组和对照组之间共鉴定出329个lncRNAs和179个mRNA存在差异表达。基因本体分析显示，10个最显著富集的功能包括上调的mRNA，最显著富集的术语与蛋白质细胞外基质（ECM）有关。在通路分析中，两个最显著富集的通路是上调mRNA的TGF-β信号通路和下调mRNA的磷酸戊糖途径。此外，对于某些失调的lncRNAs，其相关的mRNA也失调。值得注意的是，作为TGF-β通路的靶基因，骨形态发生蛋白和激活素膜结合抑制剂以及TGF-β诱导物被表明与ECM和侵袭性胎盘细胞密切相关。它们附近的lncRNAs G008916和穹窿体RNA2-1也显著失调。总之，鉴定出了具有作为AIP生物标志物潜力的重要lncRNAs。
