人脐带华通氏胶间充质干细胞通过旁分泌机制保护神经细胞免受氧化应激损伤。

Human Wharton's jelly mesenchymal stem cells protect neural cells from oxidative stress through paracrine mechanisms.

作者信息

Puig-Pijuan Teresa, de Godoy Mariana A, Pinheiro Carvalho Luiza Rachel, Bodart-Santos Victor, Lindoso Rafael Soares, Pimentel-Coelho Pedro Moreno, Mendez-Otero Rosalia

机构信息

Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, Rio de Janeiro, Brazil.

出版信息

Future Sci OA. 2020 Sep 17;6(9):FSO627. doi: 10.2144/fsoa-2020-0036.

AIM

Mesenchymal stem cells (MSCs) have neuroprotective and immunomodulatory properties, which are partly mediated by extracellular vesicles (EVs) secretion. We aimed to evaluate the effects of human Wharton's jelly-derived MSCs (WJ-MSCs) and their EVs on rat hippocampal cultures subjected to hydrogen peroxide (HO).

MATERIALS & METHODS: Hippocampal dissociated cultures were either co-cultured with WJ-MSCs or treated with their EVs prior to HO exposure and reactive oxygen species levels and cell viability were evaluated.

RESULTS

Coculture with WJ-MSCs or pre-incubation with EVs prior to the insult reduced reactive oxygen species after HO exposure. Cell viability was improved only when coculture was maintained following the insult, while EVs did not significantly improve cell viability.