Department of Urology, The Ministry Of Health, University of Health Sciences, Van Education and Research Hospital, Van 65000, Turkey.
Department of Microbiology, The Ministry Of Health, University of HealthSciences, Van Education and ResearchHospital, Van 65000, Turkey .
Urol J. 2020 Nov 23;17(6):614-619. doi: 10.22037/uj.v16i7.6030.
This study investigates the frequency of isolated microorganisms and the antimicrobial resistant pattern of inner foreskin and smegma in prepubertal children.
This comparative cross-sectional study was conducted between March and November 2019, where 132 prepubertal boys, who were scheduled to receive religious circumcisions at our outpatient clinic, were examined. The patients were divided into the following groups based on the presence of smegma in their subpreputial space: Group I (with smegma, n=58) and Group II (without smegma, n=74). Sterile stuart transport swabs (Advanced Diagnostic Research, Mediko Kimya, Turkey) were taken from the smegma or the subpreputial space (glans surface and inner foreskin) using aseptic techniques and then the swab samples were immediately transported by sterile stuart transportation for microscopy, culture identification, and antibiographic resistance testing by conventional test methods and automated systems (VITEK II, Biomerieux, France) to the Microbiological Laboratory of our hospital.
48 bacteria isolated from 39 boys in Group I comprised 28 gram-positive species (58.3%) and 20 gram-negative species (41.7%). The most commonly isolated gram-negative bacterium was Proteus mirabilis (45%) while most positive was Staphylococcus hominis (42.9%). In Group II, 68 boys had 103 bacterial isolates in the glans comprising 81 gram-positive species (78.6%) and 22 gram-negative species (21.4%). The most commonly isolated gram-negative bacterium was Proteus mirabilis (42.9%) while the most positive were Enterococcus faecalis (40.7%) and S. hominis (42.9%) Conclusion: The subpreputial space of uncircumcised boys is colonized by various types of uropathogens resistant to multidrug drugs. Smegma does not pose additional risks to microbiological colonization in children.
本研究旨在调查青春期前儿童内包皮和包皮垢中分离微生物的频率及其对抗菌药物的耐药模式。
这是一项在 2019 年 3 月至 11 月期间进行的对比性横断面研究，共有 132 名计划在我们的门诊接受宗教割礼的青春期前男孩参与了该研究。根据他们的包皮内是否有包皮垢，将患者分为以下两组：第 I 组（有包皮垢，n=58）和第 II 组（无包皮垢，n=74）。使用无菌技术从包皮垢或包皮内（龟头表面和内包皮）采集无菌斯图尔特运输拭子（先进诊断研究，Mediko Kimya，土耳其），然后立即通过无菌斯图尔特运输将拭子样本运送到我们医院的微生物实验室，进行显微镜检查、培养鉴定和抗生素耐药性测试，采用常规测试方法和自动化系统（VITEK II，生物梅里埃，法国）。
第 I 组 39 名男孩中分离出 48 株细菌，包括 28 株革兰氏阳性菌（58.3%）和 20 株革兰氏阴性菌（41.7%）。最常见的分离革兰氏阴性菌是奇异变形杆菌（45%），而最常见的阳性菌是人葡萄球菌（42.9%）。在第 II 组中，68 名男孩的龟头有 103 株细菌，包括 81 株革兰氏阳性菌（78.6%）和 22 株革兰氏阴性菌（21.4%）。最常见的分离革兰氏阴性菌是奇异变形杆菌（42.9%），而最常见的阳性菌是粪肠球菌（40.7%）和人葡萄球菌（42.9%）。
未割礼男孩的包皮内空间被多种对抗菌药物耐药的尿路病原体定植。包皮垢不会给儿童的微生物定植带来额外的风险。
