具有人血清白蛋白特异性生物发光的腔肠素类似物。

Coelenterazine Analogue with Human Serum Albumin-Specific Bioluminescence.

作者信息

Nishihara Ryo, Niwa Kazuki, Tomita Tatsunosuke, Kurita Ryoji

机构信息

National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

DAILAB, DBT-AIST International Centre for Translational and Environmental Research (DAICENTER), National Institute of Advanced Industrial Science and Technology (AIST), Central 5-41, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.

出版信息

Bioconjug Chem. 2020 Dec 16;31(12):2679-2684. doi: 10.1021/acs.bioconjchem.0c00536. Epub 2020 Nov 25.

DOI:10.1021/acs.bioconjchem.0c00536

PMID:33236887

Abstract

A synthetic luciferin comprising an imidazopyrazinone core, named HuLumino, was designed to generate specific bioluminescence with human serum albumin (HSA) in real serum samples. HuLumino was developed by attaching a methoxy-terminated alkyl chain to C-6 of coelenterazine and by eliminating a benzyl group at C-8. HSA levels were quantified within 5% error margins of an enzyme-linked immunosorbent assay without the need for any sample pretreatments because of the high specificity of HuLumino.

摘要

一种包含咪唑并吡嗪酮核心的合成荧光素，名为HuLumino，旨在在真实血清样本中与人类血清白蛋白（HSA）产生特定的生物发光。HuLumino是通过将甲氧基封端的烷基链连接到腔肠素的C-6位并去除C-8位的苄基而开发的。由于HuLumino的高特异性，无需任何样品预处理即可在酶联免疫吸附测定5%的误差范围内对HSA水平进行定量。