Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu 182-8585, Japan.
School of Life Science and Technology, Tokyo Institute of Technology, B-62 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Int J Mol Sci. 2022 Oct 27;23(21):13047. doi: 10.3390/ijms232113047.
The present study introduces a unique BL signature imaging system with novel CTZ analogues named "C-series." Nine kinds of C-series CTZ analogues were first synthesized, and BL intensity patterns and spectra were then examined according to the marine luciferases. The results show that the four CTZ analogues named C3, C4, C6, and C7, individually or collectively luminesce with completely distinctive BL spectral signatures and intensity patterns according to the luciferases: luciferase (RLuc), NanoLuc, and artificial luciferase (ALuc). The signatural reporters were multiplexed into a multi-reporter system comprising RLuc8.6-535SG and ALuc16. The usefulness of the signatural reporters was further determined with a multi-probe system that consists of two single-chain probes embedding RLuc8 and ALuc23. This study is a great addition to the study of conventional bioassays with a unique methodology, and for the specification of each signal in a single- or multi-reporter system using unique BL signatures and patterns of reporter luciferases.
本研究介绍了一种独特的 BL 签名成像系统，该系统使用新型 CTZ 类似物，名为“C 系列”。首次合成了 9 种 C 系列 CTZ 类似物，并根据海洋荧光素酶检查了 BL 强度模式和光谱。结果表明，根据荧光素酶（RLuc）、NanoLuc 和人工荧光素酶（ALuc），单独或集体发光的四种 CTZ 类似物 C3、C4、C6 和 C7，具有完全独特的 BL 光谱特征和强度模式。报告基因被多路复用成一个多报告基因系统，包括 RLuc8.6-535SG 和 ALuc16。使用包含 RLuc8 和 ALuc23 的两个单链探针的多探针系统进一步确定了报告基因的有用性。该研究为常规生物测定的研究增加了独特的方法，并为使用独特的 BL 报告基因的特征和模式在单个或多报告基因系统中指定每个信号提供了依据。
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