Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Chapel Hill, NC, 27599, USA.
Toxicity Assessment Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA.
Biometals. 2021 Feb;34(1):97-105. doi: 10.1007/s10534-020-00266-w. Epub 2020 Nov 25.
To determine the effects of repeated physical activity on iron and zinc homeostases in a living system, we quantified blood and tissue levels of these two metals in sedentary and physically active Long-Evans rats. At post-natal day (PND) 22, female rats were assigned to either a sedentary or an active treatment group (n = 10/group). The physically active rats increased their use of a commercially-constructed stainless steel wire wheel so that, by the end of the study (PND 101), they were running an average of 512.8 ± 31.9 (mean ± standard error) min/night. After euthanization, plasma and aliquots of liver, lung, heart, and gastrocnemius muscle were obtained. Following digestion, non-heme iron and zinc concentrations in plasma and tissues were measured using inductively coupled plasma optical emission spectroscopy. Concentrations of both non-heme iron and zinc in plasma and liver were significantly decreased among the physically active rats relative to the sedentary animals. In the lung, both metals were increased in concentration among the physically active animals but the change in zinc did not reach significance. Similarly, tissue non-heme iron and zinc levels were both increased in heart and muscle from the physically active group. It is concluded that repeated physical activity in an animal model can be associated with a translocation of both iron and zinc from sites of storage (e.g. liver) to tissues with increased metabolism (e.g. the lung, heart, and skeletal muscle).
为了确定反复的体力活动对活体系统中铁和锌的体内平衡的影响，我们对久坐不动和活跃的 Long-Evans 大鼠的血液和组织中的这两种金属的水平进行了定量分析。在出生后第 22 天（PND 22），雌性大鼠被分配到久坐不动或活跃治疗组（每组 n = 10）。活跃的大鼠增加了对商业制造的不锈钢丝轮的使用，以至于在研究结束时（PND 101），它们的平均夜间跑步时间为 512.8 ± 31.9（平均值 ± 标准误差）分钟。安乐死后，获得血浆和肝、肺、心脏和比目鱼肌的等分试样。消化后，使用电感耦合等离子体光学发射光谱法测量血浆和组织中非血红素铁和锌的浓度。与久坐不动的动物相比，活跃的大鼠的血浆和肝脏中的非血红素铁和锌浓度显著降低。在肺中，两种金属的浓度在活跃的动物中都增加了，但锌的变化没有达到显著性。同样，来自活跃组的心脏和肌肉中的组织非血红素铁和锌水平均增加。因此，可以得出结论，动物模型中的反复体力活动可能与铁和锌从储存部位（例如肝脏）向代谢增加的组织（例如肺、心脏和骨骼肌）的转移有关。
