Stöcker W, Otte M, Ulrich S, Normann D, Finkbeiner H, Stöcker K, Jantschek G, Scriba P C
Dept. of Medicine, Medical University of Lübeck, FRG.
Scand J Gastroenterol Suppl. 1987;139:41-52. doi: 10.3109/00365528709089774.
The sera of 59 patients with Crohn's disease (CD) and of 46 patients with ulcerative colitis (UC) were tested for autoantibodies (Aab) by indirect immunofluorescence with modern histochemical techniques using 19 different human tissues as antigenic substrates. Control collectives consisted of 19 patients with coeliac disease and of 100 healthy subjects. It was possible to demonstrate a specific marker for CD: Aab against exocrine pancreas (Pab) were present in 39% of the CD sera (UC 4%, coeliac disease 0%, healthy controls 3%). High Pab titres were only detectable in CD sera (29%). The CD-related autoantigen was demonstrated to be a component of normal pancreatic juice. Pab in CD were fundamentally different from those sometimes occurring in chronic and acute pancreatitis. It is suggested that CD is caused by autoimmune reactions against a component of pancreatic juice. Pab in CD correspond to Aab against intestinal goblet cells (Gab), which occurred exclusively in UC (28%). Pab and Gab, but obviously none of the other Aab investigated in this study, are of diagnostic value in chronic inflammatory bowel disease.
采用现代组织化学技术，以19种不同人体组织作为抗原底物，通过间接免疫荧光法对59例克罗恩病（CD）患者及46例溃疡性结肠炎（UC）患者的血清进行自身抗体（Aab）检测。对照群体包括19例乳糜泻患者和100名健康受试者。结果发现了一种CD的特异性标志物：39%的CD患者血清中存在抗外分泌胰腺的自身抗体（Pab）（UC患者中为4%，乳糜泻患者中为0%，健康对照者中为3%）。仅在CD患者血清中可检测到高滴度的Pab（29%）。已证实与CD相关的自身抗原是正常胰液的一种成分。CD患者中的Pab与慢性和急性胰腺炎中偶尔出现的Pab有根本区别。提示CD是由针对胰液一种成分的自身免疫反应引起的。CD患者中的Pab与抗肠杯状细胞的自身抗体（Gab）相对应，后者仅出现在UC患者中（28%）。Pab和Gab，显然还有本研究中检测的其他Aab，在慢性炎症性肠病中均具有诊断价值。
