褪黑素通过促进 MdWOX11 的功能促进苹果不定根的形成。
Melatonin promotes adventitious root formation in apple by promoting the function of MdWOX11.
机构信息
College of Horticulture, Northwest Agriculture & Forestry University, Yangling, 712100, China.
College of Life Science, Northwest Agriculture & Forestry University, Yangling, 712100, China.
出版信息
BMC Plant Biol. 2020 Nov 26;20(1):536. doi: 10.1186/s12870-020-02747-z.
BACKGROUND
Melatonin (MT) is important for plant growth and development; however, it is not known whether MT is involved in apple adventitious root (AR) development. In this study, we treated Malus prunifolia (MP) at four different stages of AR development, and analyzed the level of the endogenous hormones MT, auxin (IAA), zeatin-riboside (ZR), abscisic acid (ABA), and gibberellins (GA) in all four treatment groups and the untreated control group. The expression of MT, IAA biosynthesis, transport and signal transduction, the cell cycle, and root development related genes were quantified by RT-qPCR. The function of MdWOX11 was analyzed in transgenic apple plants.
RESULTS
The promotion of AR development by MT was dependent on the stage of AR induction between 0 and 2 d in apple rootstocks. MT-treatment increased the level of IAA and crosstalk existed between MT and IAA during AR formation. The expression of MdWOX11 was induced by MT treatment and positively regulated AR formation in apple. Furthermore, transgenic lines that overexpressed MdWOX11 lines produced more ARs than 'GL3'. Phenotypic analysis indicated that MdWOX11 overexpression lines were more sensitive to exogenous MT treatment than 'GL3', suggesting that MdWOX11 regulates AR formation in response to MT in apple rootstock.
CONCLUSIONS
MT promotes AR formation mainly during the AR induction stage by inducing IAA levels and upregulating MdWOX11.
背景
褪黑素（MT）对植物的生长和发育很重要；然而，目前尚不清楚 MT 是否参与了苹果不定根（AR）的发育。在本研究中，我们在苹果不定根发育的四个不同阶段处理了山荆子（MP），并分析了所有四个处理组和未处理对照组中内源性激素 MT、生长素（IAA）、玉米素核苷（ZR）、脱落酸（ABA）和赤霉素（GA）的水平。通过 RT-qPCR 定量分析了 MT、IAA 生物合成、运输和信号转导、细胞周期和根发育相关基因的表达。分析了 MdWOX11 在转基因苹果植株中的功能。
结果
MT 对不定根发育的促进作用取决于苹果砧木中不定根诱导的 0 到 2d 之间的阶段。MT 处理增加了 IAA 的水平，并且在 AR 形成过程中 MT 和 IAA 之间存在交叉对话。MdWOX11 的表达受 MT 处理诱导，并正向调节苹果的 AR 形成。此外，过表达 MdWOX11 的转基因株系比‘GL3’产生了更多的 AR。表型分析表明，与‘GL3’相比，过表达 MdWOX11 的株系对 MT 的处理更为敏感，这表明 MdWOX11 通过调节 AR 的形成来响应 MT 在苹果砧木中的作用。
结论
MT 主要通过诱导 IAA 水平和上调 MdWOX11 来促进 AR 形成，尤其是在 AR 诱导阶段。
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