National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P. R. China.
Food Funct. 2020 Dec 1;11(12):10936-10944. doi: 10.1039/d0fo01232g. Epub 2020 Nov 27.
Phosphorylation may enhance the functional properties of proteins/peptides. Herring egg phosphopeptides (HEPPs) have been found to be more effective than the non-phosphorylated variant in calcium-binding activities due to the introduced phosphate groups. However, whether HEPPs as calcium carriers will be superior to herring egg peptides (HEPs) in improving calcium bioavailability in vivo, for the equivalent calcium intake prerequisite, remains to be clarified. This study aimed to evaluate the effect of HEPPs-calcium complex and HEPs-calcium complex on calcium absorption and bioavailability in calcium-deficient mice. Results showed that the remarkably lower calcium absorption and bone calcium deposition induced by long-term calcium deficiency were accompanied by deterioration of the trabecular bone microarchitecture (P < 0.05). The HEPPs-Ca supplements significantly improved the apparent calcium absorption, increased the serum calcium level, decreased the alkaline phosphatase activity, strengthened the bone biomechanical property, and increased bone volume/tissue volume (BV/TV) and trabecular number (Tb·N) in calcium-deficient mice (P < 0.05), as determined by micro-computed tomography (micro-CT) assay. The effect of HEPPs-Ca on calcium absorption and bioavailability was comparable to that of CPPs-Ca, but better than that of HEPs-Ca and CaCO. This study brings new insights into the potential of HEPPs as an alternative to CPPs for use in calcium supplements.
磷酸化可能增强蛋白质/肽的功能特性。由于引入了磷酸基团，已发现鲱鱼卵磷酸肽（HEPPs）在钙结合活性方面比非磷酸化变体更有效。然而，在体内同等钙摄入量的前提下，作为钙载体的 HEPPs 是否在提高钙生物利用度方面优于鲱鱼卵肽（HEPs），仍需阐明。本研究旨在评估 HEPPs-钙复合物和 HEPs-钙复合物对缺钙小鼠钙吸收和生物利用度的影响。结果表明，长期缺钙引起的钙吸收和骨钙沉积显著降低，伴随着小梁骨微结构恶化（P<0.05）。HEPPs-Ca 补充剂显著提高了表观钙吸收，增加了血清钙水平，降低了碱性磷酸酶活性，增强了骨生物力学特性，并增加了缺钙小鼠的骨量/组织量（BV/TV）和小梁数（Tb·N）（P<0.05），通过微计算机断层扫描（micro-CT）检测。HEPPs-Ca 对钙吸收和生物利用度的影响与 CPPs-Ca 相当，但优于 HEPs-Ca 和 CaCO。本研究为 HEPPs 作为 CPPs 的替代品用于钙补充剂提供了新的见解。
