Laboratory for Animal Health, Bacterial Zoonosis Unit, European and OIE Reference Laboratory for Glanders, Paris-Est University, ANSES, Maisons-Alfort, France.
Veterinary Diagnostic Laboratory Centre & Animal Health Department, Agriculture Affairs & Fish Resources Authority (PAAF), Kuwait.
Transbound Emerg Dis. 2021 Mar;68(2):960-963. doi: 10.1111/tbed.13777. Epub 2020 Sep 11.
Glanders is a contagious zoonotic disease caused by Burkholderia mallei. Following the detection of glanders positive horses using the OIE complement fixation test, the tissues of two horses were analysed by PCR. While PCR systems targeting the Burkholderia pseudomallei complex gave positive signals, the species-specific PCR systems targeting B. mallei (fliP-IS407A) and B. pseudomallei (orf11)-the OIE recommended targets-resulted in negative signals. However, the presence of B. mallei in these tissues was confirmed with a recently described B. mallei-specific real-time PCR system and genotyping with MLST- and SNP-based methods, performed on the most positive tissue, identified a genotype closely related to B. mallei strains recently isolated in the Middle East. This study leads to recommendations regarding the use of PCR systems for the molecular diagnosis of glanders, especially in regions where the circulating B. mallei strains have not yet been fully genetically characterized.
类鼻疽是由鼻疽伯克霍尔德菌引起的一种传染性人畜共患病。在使用世界动物卫生组织补体结合试验检测到阳性马后，对两匹马的组织进行了 PCR 分析。虽然针对伯克霍尔德氏菌复合体的 PCR 系统给出了阳性信号，但针对鼻疽伯克霍尔德菌（fliP-IS407A）和鼻疽伯克霍尔德菌（orf11）的种特异性 PCR 系统（世界动物卫生组织推荐的靶标）的结果为阴性信号。然而，最近描述的一种针对鼻疽伯克霍尔德菌的特异性实时 PCR 系统和基于 MLST 和 SNP 的基因分型方法，对最阳性组织进行分析，证实了这些组织中存在鼻疽伯克霍尔德菌。从中东最近分离到的鼻疽伯克霍尔德菌菌株。本研究对用于类鼻疽分子诊断的 PCR 系统的使用提出了建议，特别是在尚未对循环的鼻疽伯克霍尔德氏菌菌株进行充分遗传特征分析的地区。
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