Somov Institute of Epidemiology and Microbiology.
Klin Lab Diagn. 2020 Sep 17;65(10):659-664. doi: 10.18821/0869-2084-2020-65-10-659-664.
In the territory of the Primorsky region, the nosoareas of causative agents of tick-borne infections often coincide and lead to mixed infection of ticks, which causes the mixed pathology in humans . We investigated ixodid ticks taken from people during the epidemic season of 2019 for the spectrum of pathogens of transmissible infections to determine the degree of their mono- and mixed infection. 651 specimens of ixodid ticks investigated. RNA/DNA pathogens was determined by the method of polymerase chain reaction (PCR), TBEV antigen by ELISA. The presence in ticks of pathogens of the following diseases was detected: Lyme borreliosis (35.3%), tick-borne replasing fever (11.3%), human granulocytic anaplasmosis (2.6%), human monocytic ehrlichiosis (2.4%), tick-borne encephalitis (1.8%), tick-borne ricketsiosis (1.0%). As agents of mono-infections, the virus of TBE, ehrlichia, anaplasma and rickettsia in ticks occurred less frequently than in combination with other pathogens. A high frequency of occurrence B. burgdorferi s.l. in mono-infection detected (58.7%). B. miyamotoi has met evenly at ticks with mono- and mixed infections. Total mixed infection of ticks was 23.1%. Borrelia have been identified in all mixed cases. Variants of double combinations (95.5 % of cases from all mixed infections) were as follows: B. burgdorferi s. l. + A. phagocytophilum, B. burgdorferi s.l. + E. chaffeensis / E. muris, B. burgdorferi s.l. + TBEV, B. burgdorferi s.l. + R. heilongjiangensis, B. burgdorferi s.l. + B. miyamotoi. The most frequent combination was B. burgdorferi s.l. + B. miyamotoi (37.8% of all mixed infections). In triple infection of B. burgdorferi s.l. + A. phagocytophilum + TBEV and B. burgdorferi s.l. + A. phagocytophilum + B. miyamotoi, the frequency of occurrence for each combination was 2.2%. Given the above, a complex laboratory diagnosis of tick-borne infections in individuals who have been bitten by a tick is necessary, including the detection of all pathogens common in focal areas.
在滨海边疆区境内，蜱传感染病原体的疫源地经常重合，导致蜱混合感染，这会导致人类混合性疾病。我们调查了在 2019 年流行季节从人身上采集的硬蜱，以确定可传播感染病原体的范围，以确定其单重和混合感染的程度。共调查了 651 份硬蜱标本。采用聚合酶链反应（PCR）方法检测 RNA/DNA 病原体，采用 ELISA 法检测 TBEV 抗原。在蜱中检测到以下疾病的病原体：莱姆病（35.3%）、蜱传回归热（11.3%）、人粒细胞无形体病（2.6%）、人单核细胞埃立克体病（2.4%）、乙型脑炎（1.8%）、蜱传斑疹伤寒（1.0%）。作为单重感染的病原体，TBE 病毒、埃立克体、无形体和立克次体在蜱中的发生率低于与其他病原体的组合。在单重感染中，检测到伯氏疏螺旋体 s.l. 的高发生率（58.7%）。在单重和混合感染中均均匀检测到 B. miyamotoi。蜱的总混合感染率为 23.1%。在所有混合病例中均鉴定出伯氏疏螺旋体。双重组合的变体（所有混合感染病例的 95.5%）如下：伯氏疏螺旋体 s.l.+ A. phagocytophilum、伯氏疏螺旋体 s.l.+ E. chaffeensis/E. muris、伯氏疏螺旋体 s.l.+ TBEV、伯氏疏螺旋体 s.l.+ R. heilongjiangensis、伯氏疏螺旋体 s.l.+ B. miyamotoi。最常见的组合是伯氏疏螺旋体 s.l.+ B. miyamotoi（所有混合感染的 37.8%）。在伯氏疏螺旋体 s.l.+ A. phagocytophilum+TBEV 和伯氏疏螺旋体 s.l.+ A. phagocytophilum+B. miyamotoi 的三重感染中，每种组合的发生率均为 2.2%。鉴于上述情况，有必要对被蜱叮咬的个体进行复杂的蜱传感染实验室诊断，包括检测在重点地区常见的所有病原体。
